おすすめの製品
product name
白血病抑制因子, human, recombinant, expressed in HEK 293 cells
由来生物
human
品質水準
リコンビナント
expressed in HEK 293 cells
アッセイ
≥95% (SDS-PAGE)
形状
liquid
有効性
≤0.5 ng/mL M1 cells proliferation inhibition EC50
分子量
20 kDa
包装
pkg of 10 μg
テクニック
cell culture | mammalian: suitable
不純物
≤1 EU/μg protein Endotoxin level
UniProtアクセッション番号
保管温度
−20°C
遺伝子情報
human ... LIF(3976)
詳細
LIF is manufactured using an all-human production system, with no serum.
Leukemia Inhibitory Factor (LIF) gene is mapped on the human chromosome at 22q12.2.
Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.
アプリケーション
Leukemia inhibitory factor (LIF) has been used to evaluate its effect on embryo development and aneuploidy in matured (IVM) human oocytes, in vitro.
One of the main uses of LIF in research is in mouse embryonic stem cell culture maintenance. LIF is necessary to maintain the stem cells in an undifferentiated state while retaining their proliferative potential or pluripotency. Removal of LIF pushes stem cells toward differentiation.
生物化学的/生理学的作用
Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts. It also inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B cell lymphoma) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.
物理的形状
This product is supplied as a solution in 0.2 μm filtered phosphate buffered saline with no additives or carrier proteins. It is aseptically filled.
調製ノート
Briefly centrifuge the vial before opening. After initial thawing it is recommended to store the protein in working aliquots at -20°C. The product can be diluted in PBS.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SRP9001-10UG-PW:
SRP9001-10UG:
SRP9001-VAR:
SRP9001-BULK:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
この製品を見ている人はこちらもチェック
Protective effects of leukemia inhibitory factor against oxidative stress during high glucose-induced apoptosis in podocytes.
Cell Stress & Chaperones, 17(4), 485-493 (2012)
Cytokines hold promise for human embryo culture in vitro: results of a randomized clinical trial
Fertility and Sterility, 112(5), 849-857 (2019)
Leukemia inhibitory factor (LIF) and its biological activity.
Annales Universitatis Mariae Curie-Sklodowska. Sectio D: Medicina, 59(2), 189-193 (2004)
The gp130 receptor cytokine family: regulators of adipocyte development and function.
Current Pharmaceutical Design, 17(4), 340-346 (2011)
Complex conserved organization of the mammalian leukemia inhibitory factor gene: regulated expression of intracellular and extracellular cytokines.
Journal of Immunology, 162(8), 4637-4646 (1999)
資料
Read article on hematopoietic cytokines and hematopoiesis
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)