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Merck

SRP9001

Sigma-Aldrich

Leukemia Inhibitory Factor human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, liquid, suitable for cell culture

別名:

LIF

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About This Item

UNSPSCコード:
12352202
NACRES:
NA.32

product name

白血病抑制因子, human, recombinant, expressed in HEK 293 cells

由来生物

human

品質水準

リコンビナント

expressed in HEK 293 cells

アッセイ

≥95% (SDS-PAGE)

形状

liquid

有効性

≤0.5 ng/mL M1 cells proliferation inhibition EC50

分子量

20 kDa

包装

pkg of 10 μg

テクニック

cell culture | mammalian: suitable

不純物

≤1 EU/μg protein Endotoxin level

UniProtアクセッション番号

保管温度

−20°C

遺伝子情報

human ... LIF(3976)

詳細

LIF is manufactured using an all-human production system, with no serum.
Leukemia Inhibitory Factor (LIF) gene is mapped on the human chromosome at 22q12.2.
Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.

アプリケーション

Leukemia inhibitory factor (LIF) has been used to evaluate its effect on embryo development and aneuploidy in matured (IVM) human oocytes, in vitro.
One of the main uses of LIF in research is in mouse embryonic stem cell culture maintenance. LIF is necessary to maintain the stem cells in an undifferentiated state while retaining their proliferative potential or pluripotency. Removal of LIF pushes stem cells toward differentiation.

生物化学的/生理学的作用

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts. It also inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B cell lymphoma) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.

物理的形状

This product is supplied as a solution in 0.2 μm filtered phosphate buffered saline with no additives or carrier proteins. It is aseptically filled.

調製ノート

Briefly centrifuge the vial before opening. After initial thawing it is recommended to store the protein in working aliquots at -20°C. The product can be diluted in PBS.

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SRP9001-10UG-PW:
SRP9001-10UG:
SRP9001-VAR:
SRP9001-BULK:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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Protective effects of leukemia inhibitory factor against oxidative stress during high glucose-induced apoptosis in podocytes.
Xu J et al
Cell Stress & Chaperones, 17(4), 485-493 (2012)
Cytokines hold promise for human embryo culture in vitro: results of a randomized clinical trial
Fawzy M, et al.
Fertility and Sterility, 112(5), 849-857 (2019)
Leukemia inhibitory factor (LIF) and its biological activity.
Chodorowska G et al
Annales Universitatis Mariae Curie-Sklodowska. Sectio D: Medicina, 59(2), 189-193 (2004)
The gp130 receptor cytokine family: regulators of adipocyte development and function.
White UA and Stephens JM
Current Pharmaceutical Design, 17(4), 340-346 (2011)
Complex conserved organization of the mammalian leukemia inhibitory factor gene: regulated expression of intracellular and extracellular cytokines.
Haines BP et al
Journal of Immunology, 162(8), 4637-4646 (1999)

資料

Read article on hematopoietic cytokines and hematopoiesis

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