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由来生物
human
リコンビナント
expressed in HEK 293 cells
タグ
6-His tagged (C-terminus)
アッセイ
≥95% (SDS-PAGE)
形状
lyophilized
分子量
calculated mol wt 30 kDa
observed mol wt 30 kDa (DTT-reduced. Ser 2 is the predicted N-terminal .)
包装
pkg of 10 μg
pkg of 50 μg
不純物
<1 EU/μg endotoxin (LAL test)
UniProtアクセッション番号
輸送温度
wet ice
保管温度
−20°C
遺伝子情報
human ... CA2(760)
詳細
Carbonic Anhydrase 2 (CA2) is a zinc metalloenzyme and it helps in regulating acid-base balance in cells. It is a ubiquitous cytosolic protein. Carbonic anhydrase 2 also known as carbonate dehydratase II, carbonic anhydrase C, belongs to the class of a carbonic anhydrases. The associated gene is mapped to human chromosome 8q21.
アプリケーション
Carbonic Anhydrase 2/CA2 human has been used for CA2 ELISA (enzyme-linked immunosorbent assay).
生物化学的/生理学的作用
Carbonic Anhydrase 2 (CA2) catalyzes the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa). CA2 can bind to various transporters, such as anion exchanger 1 (AE1), NBCe1 (Na(+)/HCO3(-) cotransporter), NHE3 (Na(+)/H(+) exchanger 3) and MCT (monocarboxylate transporter). This association increases the rate of transport. Mutations in the CA2 gene are responsible for carbonic anhydrase II (CAII) deficiency syndrome, an autosomal recessive osteopetrosis characterised with renal tubular acidosis as well as cerebral calcifications. Changes in the expression of CA2 are linked with carcinogenesis.
物理的形状
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT. Normally Mannitol or Trehalose is added as protectants before lyophilization.
再構成
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Eye Irrit. 2
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SRP6484-10UG:
SRP6484-50UG:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Characterization of CA XIII, a novel member of the carbonic anhydrase isozyme family.
The Journal of Biological Chemistry, 279, 2719-2719 (2004)
Carbonic anhydrase II binds to and increases the activity of the epithelial sodium-proton exchanger, NHE3.
American Journal of Physiology: Renal Physiology, 309, F383-F383 (2015)
Carbonic anhydrase 2-like a and 15a are involved in acid-base regulation and Na+ uptake in zebrafish H+-ATPase-rich cells.
American Journal of Physiology. Cell Physiology, 294, C1250-C1250 (2008)
Amplification of chromosome 8q21-qter associated with the acquired paclitaxel resistance of nasopharyngeal carcinoma cells.
International Journal of Clinical and Experimental Pathology, 8, 12346-12346 (2015)
Increased water flux induced by an aquaporin-1/carbonic anhydrase II interaction.
Molecular Biology of the Cell, 26, 1106-1106 (2015)
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