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由来生物
human
リコンビナント
expressed in HEK 293 cells
タグ
6-His tagged (C-terminus)
アッセイ
≥95% (SDS-PAGE)
形状
lyophilized
分子量
calculated mol wt 29 kDa (after removal of signal peptide.)
observed mol wt 33-36 kDa by SDS-PAGE
包装
pkg of 10 and 50 μg
不純物
<1 EU/μg endotoxin (LAL test)
UniProtアクセッション番号
輸送温度
wet ice
保管温度
−20°C
遺伝子情報
human ... EPCAM(4072)
詳細
DTT-reduced. rh EpCAMProtein migrates as poly peptide due to glycosylation. Gln24 is the predicted N-terminal.
Epithelial cell adhesion molecule (EpCAM) is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A (mAb) antigen. The gene encoding EpCAM is localized on human chromosome 2p21.
生物化学的/生理学的作用
Epithelial cell adhesion molecule (EpCAM) is intricately linked with the cadherin-catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The protein has several cellular functions, like epithelial-specific cell-adhesion, cell signaling, migration, proliferation and differentiation. It is expressed in most epithelial malignancies (like epithelial ovarian cancer) and was reported as a tumor marker or a candidate of molecular targeting therapy. EpCAM cross signaling with N-cadherin involves phosphoinositide-3-kinase (Pi3K), resulting in the abrogation of the cadherin adhesion complexes in epithelial cells.
物理的形状
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
再構成
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SRP6455-10UG:
SRP6455-50UG:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Overexpression of epithelial cell adhesion molecule (Ep-CAM) is an independent prognostic marker for reduced survival of patients with epithelial ovarian cancer.
Gynecologic Oncology, 103(2), 483-488 (2006)
Cadherins are regulated by Ep-CAM via phosphaditylinositol-3 kinase.
Molecular and Cellular Biochemistry, 302(1-2), 19-26 (2007)
Epithelial cell adhesion molecule expression (CD326) in cancer: a short review.
Cancer Treatment Reviews, 38(1), 68-75 (2012)
The evolving roles of canonical WNT signaling in stem cells and tumorigenesis: implications in targeted cancer therapies
Laboratory Investigation; a Journal of Technical Methods and Pathology, 96, 116-136 (2016)
The Epithelial Cell Adhesion Molecule (Ep-CAM) as a Morphoregulatory Molecule Is a Tool in Surgical Pathology
The American Journal of Pathology, 163(6), 2139-2148 (2003)
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