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リコンビナント
expressed in baculovirus infected Sf9 cells
製品種目
PRECISIO® Kinase
アッセイ
≥70% (SDS-PAGE)
フォーム
buffered aqueous glycerol solution
比活性
157-213 nmol/min·mg
分子量
~59 kDa (CDK5)
~60 kDa (p35)
テクニック
activity assay: suitable
溶解性
soluble
water: soluble
輸送温度
dry ice
保管温度
−70°C
遺伝子情報
human ... CDK5(1020) , CDK5R1(8851)
詳細
Research area: Apoptosis
Cdk5 is a proline-directed serine/threonine protein kinase that belongs to the family of cyclin-dependent kinases (CDKs). Active CDK5, also known as neuronal cdc2-like kinase is a heterodimer of CDK5 and is essential for its kinase activity. They are found abundantly in the mammalian brain.
Cdk5 is a proline-directed serine/threonine protein kinase that belongs to the family of cyclin-dependent kinases (CDKs). Active CDK5, also known as neuronal cdc2-like kinase is a heterodimer of CDK5 and is essential for its kinase activity. They are found abundantly in the mammalian brain.
アプリケーション
CDK5 has been used as a component of a buffer during the measurement of the in vitro phosphorylation of peroxisome proliferator-activated receptor gamma (PPARγ).
生物化学的／生理学的作用
CDK5 is a neuron-specific kinase that plays a vital role in the development, functioning, and diseases of the central nervous system. Its optimum functioning is highly essential for proper neuronal migration, differentiation, axonal elongation, and synaptic function. CDK5 modulates neurite outgrowth, and axonal growth by interacting with several substrates like N- cadherin, Rap Guanine Nucleotide Exchange Factor 2(RapGEF2), focal adhesion kinase (FAK), p21 activating kinase 1, serotonin 6 receptor and Ras-related protein 8. CDK5 also plays a key role in the formation of the synapse, its maintenance, and communication. It is involved in the formation and retraction of the dendritic spines. It also regulates neuron secretion at the synapse by phosphorylating key mediators such as Synaspsin1. In addition, CDK5, present in the synaptic membrane phosphorylates the erythroblastic leukemia viral oncogene homolog B receptor (ErbB), resulting in increased acetylcholine receptor transcription. Lastly, CDK5 is also involved in the regulation of various other cellular processes like cell cycle, cell survival, apoptosis, gene regulation, etc.
物理的形状
50 mM Tris-HCl(pH 7.5、150 mM NaCl、10 mMグルタチオン、0.1 mM EDTA、0.25 mM DTT、0.1 mM PMSF、25%グリセロール)の溶液として調製されています。
調製ノート
開封後は少量に分注して-70°Cで保存してください。繰り返して使用したり、何回も凍結融解をしないでください。
法的情報
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
カルタヘナ法
カルタヘナ法
Jan Code
SRP5011-10UG:
最新バージョンのいずれかを選択してください：
R Dhavan et al.
Nature reviews. Molecular cell biology, 2(10), 749-759 (2001-10-05)
Since it was identified a decade ago, cyclin-dependent kinase 5 (CDK5) has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a
D Tang et al.
Progress in cell cycle research, 2, 205-216 (1996-01-01)
While cyclin-dependent kinase 5 (Cdk5) is widely distributed in mammalian tissues and in cultured cell lines, Cdk5-associated kinase activity has been demonstrated only in mammalian brains. An active form of Cdk5, called neuronal cdc2-like kinase (Nclk) has been purified from
Franck Peiretti et al.
Journal of medicinal chemistry, 63(21), 13124-13139 (2020-11-04)
A proprietary library of novel N-aryl-substituted amino acid derivatives bearing a hydroxamate head group allowed the identification of compound 3a that possesses weak proadipogenic and peroxisome proliferator-activated receptor γ (PPARγ) activating properties. The systematic optimization of 3a, in order to
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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