SRP0364

mTOR/Raptor/MLST8 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥50% (SDS-PAGE)

• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected Sf9 cells
• アッセイ: ≥50% (SDS-PAGE)
• 形状: aqueous solution
• 分子量: 137 kDa (mTOR); 150 kDa (Raptor); 37 kDa (MLST8)
• 包装: pkg of 10 μg
• メーカー／製品名: Sigma-Aldrich
• テクニック: cell based assay: suitable
• 溶解性: water: soluble
• NCBIアクセッション番号: NM_004958 (mTOR); NM_020761 (Raptor); NM_022372 (MLST8)
• アプリケーション: life science and biopharma
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... MLST8(64223) , MTOR(2475) , RPTOR(57521)

詳細

詳細

This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human mTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.

アプリケーション

Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions.

生物化学的／生理学的作用

Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. mTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory-associated protein of mTOR complex 1 (RAPTOR) has an important role in modulating cell growth and energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of the mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable