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由来生物
bacterial
品質水準
フォーム
lyophilized powder
比活性
≥1000 U/mg
特徴
DNA free
テクニック
DNA extraction: suitable
適合性
suitable for cell lysis
アプリケーション
cell analysis
輸送温度
ambient
保管温度
−20°C
詳細
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases. This product is free of detectable microbial DNA and is suitable for microbiome research
アプリケーション
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
生物化学的/生理学的作用
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. Optimum pH is 8.5-9.
特徴および利点
Contains two bacteriolytic proteases for efficient lysis of Gram-positive bacteria and Gram-negative organisms
Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods
Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA
Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies
Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods
Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA
Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies
単位の定義
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Resp. Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAE0196-BULK:
SAE0196-VAR:
SAE0196-50MG:
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