SAB4504337

Anti-phospho-ASK1 (pSer⁹⁶⁶) antibody produced in rabbit

affinity isolated antibody

• UNSPSCコード: 12352203
• NACRES: NA.41
• クローン: polyclonal
• application: ELISA; IHC; WB
• 化学種の反応性: human, rat, mouse
• テクニック: ELISA: 1:20000; immunohistochemistry: 1:50-1:100; western blot: 1:500-1:1000
• citations: 10

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 154 kDa
• 化学種の反応性: human, rat, mouse
• 濃度: ~1 mg/mL
• テクニック: ELISA: 1:20000; immunohistochemistry: 1:50-1:100; western blot: 1:500-1:1000
• NCBIアクセッション番号: Q99683
• UniProtアクセッション番号: Q99683
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: phosphorylation (pSer966)
• 遺伝子情報: human ... MAP3K5(4217)

詳細

詳細

Apoptosis signal-regulating kinase 1 (ASK1) is a serine/threonine protein kinase. It belongs to the MAPKKK (mitogen-activated protein kinase) family. This gene is located on human chromosome 6q23.

Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as apoptosis signal-regulating kinase 1 (ASK1), is encoded by the gene mapped to human chromosome 6q22.33. The encoded protein belongs to the mitogen-activated protein kinase family. Porcine MAP3K5, which shares 97 % sequence identity with human MAP3K5, is characterized with a domain of unknown function (DUF4071) and a protein kinase domain.

免疫原

The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966.

Immunogen Range: 932-981

アプリケーション

Anti-phospho-ASK1 (pSer966) antibody has been used in western blotting.

Anti-phospho-ASK1 (pSer966) antibody produced in rabbit has been used in immunoblot analysis.

生物化学的／生理学的作用

Apoptosis signal-regulating kinase 1 (ASK1) is linked with apoptosis in cancer and heart tissues. It act as an upstream regulator of p38 and JNK (c-Jun N-terminal kinase) in cardiomyocytes death. Mutations in MAP3K5 gene result in β-thalassemia disease severity and hydroxyurea treatment efficacy.

Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), is one of the vital genes involved in the mitogen-activated protein kinase (MAPK) signaling cascade, which plays a crucial role in the cytoplasmic transduction of extracellular signals. Apoptosis signal-regulating kinase 1 (ASK1)-dependent mitochondrial apoptosis pathway, activated by 4SC-202, which is a novel class I HDAC inhibitor (HDACi), potentially stops hepatocellular carcinoma (HCC) cell proliferation. The encoded protein in response to oxidative stress, endoplasmic reticulum stress, infection and calcium influx plays a vital role in intracellular signaling pathways and biological functions including cell death, differentiation and inflammation. Genomic variation in MAP3K5 indicates β-thalassemia disease severity and hydroxyurea (HU) treatment efficiency. ASK1 plays a vital role in inhibition of the adipocytic differentiation process of the tumor cells and functions as an efficient therapeutic target for several human diseases including malignant fibrous histiocytomas.

特徴および利点

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物理的形状

ウサギIgGのPBS溶液（Mg2+およびCa2+を含まず）、pH 7.4、150 mM NaCl、0.02％ アジ化ナトリウム、50％ グリセロール

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable