MABC605

Anti-TXNIP Antibody, clone 3A7.1

clone 3A7.1, from mouse

• 別名: Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 3A7.1, monoclonal
• 化学種の反応性: mouse, human
• テクニック: immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2bκ
• NCBIアクセッション番号: NP_006463
• UniProtアクセッション番号: Q9H3M7
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TXNIP(10628)

詳細

詳細

Thioredoxin-interacting protein (UniProt Q9H3M7; also known as Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1) is encoded by the TXNIP (also known as VDUP1) gene (Gene ID 10628) in human. Thioredoxin interacting protein (TXNIP) binds thioredoxin active cysteine residue and suppresses its antioxidant activity. TXNIP overexpression results in increased cellular ROS level and oxidative cell death, while TXNIP suppression enhances cellular resistance to oxidative injury. TXNIP is also shown to inhibit cellular glucose uptake by downregulating glucose transporter 1 (GLUT1) plasma membrane localization, and TXNIP expression is found to be markedly suppressed in many cancers, making TXNIP a potential anticancer therapeutic target.

特異性

Clone 3A7.1 targets an epitope within the N-terminal half of TXNIP. Cross-reactivity to human and mouse spliced isoform 2 is likely, but has not been confirmed.

免疫原

GST-tagged recombinant fragment from the N-terminal half of human TXNIP.

アプリケーション

Anti-TXNIP Antibody, clone 3A7.1 is an antibody against TXNIP for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected TXNIP in HUVECs, A431, HeLa, and NIH/3T3 cells.

品質

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TXNIP in 10 µg of K562 cell lysate.

ターゲットの説明

~44 kDa observed. 43.66/37.32 kDa (human isoform 1/2) and 44.36/44.29 kDa (mouse isoform 1/2) calculated.

物理的形状

Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable