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由来生物
mouse
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
GT-34, monoclonal
形状
buffered aqueous solution
化学種の反応性
goat, sheep
包装
antibody small pack of 25 μL
濃度
~1 mg/mL
テクニック
indirect ELISA: 0.25-0.5 μg/mL using 5μg/ml Goat IgG for coating.
アイソタイプ
IgG1
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
詳細
Goat and sheep antibodies are widely used as primary antibodies against many antigens in various assay techniques in research and clinical practices. Polyclonal second antibodies to goat and sheep may suffer from a lack of unique specificity to goat or sheep IgGs, and may cross react with other IgGs from human, mouse, rat or rabbit origin. As a result, an extensive adsorbing stage has to be integrated in the manufacturing process of most polyclonal antibodies. The use of a monoclonal antibody specific to goat or sheep IgG which is devoid of any binding capacity to human and many other species, can therefore serve as an essential tool and a useful control in many tests and applications.
Monoclonal Anti-Goat/sheep IgG (mouse IgG1 isotype) is derived from the GT-34 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified goat immunoglobulins (IgGs). Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.
特異性
Monoclonal Anti-Goat/sheep recognizes an epitope located on the heavy chain of goat IgG1 and IgG2. The antibody shows strong cross-reactivity with sheep IgG and weak cross-reactivity with bovine IgG. No cross-reactivity is observed with IgG derived from human, monkey, guinea pig, rat, horse, fish, donkey, dog, chicken, pig, mouse, rabbit or cat origin. The antibody is recommended to use in various immunological techniques, including ELISA, Immunoblotting, immunofluorescence and Immunohistochemistry.
免疫原
purified goat immunoglobulins (IgGs).
生物化学的/生理学的作用
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
物理的形状
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
その他情報
This product is for R&D use only, not for drug, household, or other uses.
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保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAB4200804-25UL:
SAB4200804-BULK:
SAB4200804-100UL:
SAB4200804-VAR:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Molecular Genetics of Immunoglobulin, 17 (1987)
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The Laboratory Rat (1998)
The Immunoglobulins: Structure and Function (1998)
International journal for parasitology, 48(5), 395-402 (2018-03-15)
Some nematode species are economically important parasites of livestock, while others are important human pathogens causing some of the most important neglected tropical diseases. In both humans and animals, anthelmintic drug administration is the main control strategy, but the emergence
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