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Merck

F1763

Sigma-Aldrich

抗ラットIgG (全分子)–FITC ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

別名:

Rabbit Anti-Rat IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.46

由来生物

rabbit

品質水準

結合体

FITC conjugate

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

secondary antibodies

クローン

polyclonal

形状

buffered aqueous solution

保管条件

protect from light

テクニック

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
indirect immunofluorescence: 1:320

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

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詳細

Anti-Rat IgG (whole molecule) is produced in rabbit using purified rat IgG as the immunogen.
Rat IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rat serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Anti-Rat IgG (whole molecule)-FITC antibody binds to all rat immunoglobulins.

特異性

Rabbit polyclonal anti-Rat IgG (whole molecule)−FITC antibody reacts with rat IgG in vitro, in rat serum and biological fluids.

免疫原

Purified rat IgG

アプリケーション

Anti-Rat IgG (whole molecule)-FITC antibody has been used:
  • in immunolabelling for confocal laser scanning microscopy (CLSM)
  • in immunostaining

Anti-Rat IgG (whole molecule)-FITC antibody is suitable for use in flow cytometry (1:100) and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Rabbit polyclonal anti-Rat IgG (whole molecule)-FITC antibody may be used to detect and quantitate the level of IgG in rat serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody.

生物化学的/生理学的作用

IgGs are glycoprotein antibodies that modulate several immune responses. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.

その他情報

ヒトIgGで吸収済み抗体。

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。

調製ノート

ヒトサンプルによるバックグラウンドの染色を低減するために吸収済み。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

nwg

個人用保護具 (PPE)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

F1763-VAR:
F1763-BULK:
F1763-2ML:
F1763-1ML:
F1763-.5ML:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Structural network of arabinogalactan proteins (AGPs) and pectins in apple fruit during ripening and senescence processes
Leszczuk A, et al.
Plant Science, 275, 36-48 (2018)
Yang Zhou et al.
Experimental and therapeutic medicine, 12(4), 2671-2680 (2016-10-05)
Traditional medicinal plants are widely used as immunomodulatory medicines that help improve health. A total of 50 different plants used for the treatment of toxicity were screened for their in vivo protective effects. Flies were fed a standard cornmeal-yeast medium
Dhika Amanda et al.
Plant physiology, 172(4), 2204-2218 (2016-10-21)
The plant epidermis is crucial to survival, regulating interactions with the environment and controlling plant growth. The phytocalpain DEFECTIVE KERNEL1 (DEK1) is a master regulator of epidermal differentiation and maintenance, acting upstream of epidermis-specific transcription factors, and is required for
Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage
Zhou Y, et al.
Experimental and Therapeutic Medicine, 12(4), 2671-2680 (2016)
Martin Xaver et al.
PLoS genetics, 9(12), e1004067-e1004067 (2014-01-05)
Repairing broken chromosomes via joint molecule (JM) intermediates is hazardous and therefore strictly controlled in most organisms. Also in budding yeast meiosis, where production of enough crossovers via JMs is imperative, only a subset of DNA breaks are repaired via

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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