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由来生物
mouse
品質水準
結合体
peroxidase conjugate
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
secondary antibodies
クローン
GT-34, monoclonal
形状
lyophilized powder
化学種の反応性
sheep, goat, bovine
以下との反応禁止
rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human
包装
vial of 0.5 mL
テクニック
direct ELISA: 1:30,000
dot blot: 1:160,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
アイソタイプ
IgG1
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
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詳細
本抗体はヤギIgG1およびIgG2のH鎖に位置するエピト-プと反応します。
IgG is the most abundant antibody in human serum.It is found in blood and secreted by the B cells. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
IgGs are glycoprotein antibodies that modulate several immune responses. Goat or sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat/sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.
アプリケーション
ELISA using HRP-conjugated monoclonal anti-goat/sheep IgG as the secondary was performed on human T cells.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody has been used for western blot (1:5,000) and ELISA. The product can also be used for dot blot (1:30,000) and IHC (1:100).
生物化学的／生理学的作用
IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG enables transplacental transport. It has the longest serum half life. It might be associated with allergy.
物理的形状
Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT
調製ノート
Avrameas, S.らによって示された2段階グルタルアルデヒド法により調製しました (Scand. J. Immunol., 8, Suppl. 7, 7 (1978).)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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シグナルワード
Warning
危険有害性情報
危険有害性の分類
Skin Sens. 1
保管分類コード
13 - Non Combustible Solids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A9452-1VL:
A9452-VAR:
A9452-1VL-PW:
IXO97714:
A9452-BULK:
試験成績書（COA）
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