SAB4200757

Anti-PLA2R1 Antibody

mouse monoclonal, 12-6-5

• 別名: Anti-180 kDa secretory phospholipase A2 receptor, Anti-C-type lectin domain family 13 member C, Anti-M-type receptor, Anti-PLA2-R, Anti-PLA2R, Anti-Secretory phospholipase A2 receptor
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 製品名: Anti-PLA2R antibody, Mouse monoclonal, clone 12-6-5, purified from hybridoma cell culture
• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 12-6-5, monoclonal
• フォーム: buffered aqueous solution
• 分子量: ~180 kDa
• 交差性: human
• 濃度: ~1 mg/mL
• テクニック: flow cytometry: suitable; immunoblotting: 1-2 μg/mL using partial recombinant human PLA2R1 protein (predicted ~150kDa); immunofluorescence: suitable; immunohistochemistry: suitable
• アイソタイプ: IgG2b
• UniProtアクセッション番号: Q13018
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PLA2R1(22925)

詳細

詳細

Anti-PLA2R antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the 12-6-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with recombinant fragment of human PLA2R corresponding to extracellular sequence NC3. Secretory phospholipase A2 receptor (PLA2R) is 180 kDa and is a type I transmembrane glycoprotein member of the mannose receptor (MR) family. Large extracellular glycosylated region comprising an N-terminal cysteine-rich domain (CysR), a fibronectin-like type II domain (FnII), and eight to ten C-type lectin-like domains (CTLD).

免疫原

Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3)

アプリケーション

Anti-PLA2R antibody has been used in

• immunoblotting
• immunofluorescence
• flow Cytometry
• immunohistochemistry

生物化学的／生理学的作用

Secretory phospholipase A2 receptor (PLA2R) acts as a receptor for secretory phosholipase A2 (sPLA2) allowing its removal from circulation, thus regulating its biological effect. PLA2R is also involved in clearance followed by suppression of their potent enzymatic activities of secretory phospholipase A2 (sPLA2) from the blood, including group sPLA2-X and a snake venom sPLA2. The soluble circulating form of PLA2R is constitutively present as an endogenous inhibitor of sPLA2s. PLA2R is expressed in kidney podocytes of normal human glomeruli, in idiopathic membranous nephropathy (IMN) patients PLA2R is colocalized to immune deposits in glomeruli along with IgG4.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable