SAB4200747

Anti-Neurofilament 200 antibody, Mouse monoclonal

clone NE14, purified from hybridoma cell culture

• 別名: Anti-H-subunit, Anti-NF-H
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: NE14, monoclonal
• 形状: buffered aqueous solution
• 分子量: ~200 kDa
• 化学種の反応性: feline, rat, guinea pig, pig, human, mouse, bovine, chicken
• 濃度: ~1.0 mg/mL
• テクニック: immunoblotting: 1.25-2.5 μg/mL using rat brain S1 fraction; immunohistochemistry: 5-10 μg/mL using enzyme treated formalin-fixed, paraffin-embedded rat brain or mouse brain sections
• アイソタイプ: IgG1
• UniProtアクセッション番号: P12036
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: bovine ... Nefh(528842) ; cat ... Nefh(101087272) ; chicken ... Nefh(417020) ; human ... NEFH(4744) ; mouse ... Nefh(380684) ; pig ... Nefh(100156492) ; rat ... Nefh(24587)

詳細

詳細

Anti-Neurofilament 200 Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NE14 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are built from three intertwined protofibrils of apparent molecular weights [68 (L), 160 (M) and 200 (H) kDa] which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 200 also known as neurofilament heavy polypeptide (Hsubunit), NF-H, NEFH or 200 kDa neurofilament protein.

免疫原

Neurofilaments purified from pig spinal cord

アプリケーション

Anti-Neurofilament 200 antibody, Mouse monoclonal has been used in immunoblotting and immunohistochemistry staining.

生物化学的／生理学的作用

Neurofilaments are the type of intermediate filaments (IFs), that serve as major elements of the cytoskeleton supporting the axon cytoplasm of neuronal cells. It has an important function in mature axons that is not sub served by the two smaller neurofilament proteins. Defects in Neurofilament 200 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) and these accumulations are a hallmark of pathological lesion. Neurofilaments can accumulate in large numbers within cell bodies and proximal axons of affected neurons in several pathological diseases, such as Charcot-Marie-Tooth (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, neurofilament accumulations was detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable