SAB4200679

Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody produced in mouse

clone 1A4, purified from hybridoma cell culture

• 別名: AAT6, ACTSA, MYMY5, actin, alpha 2, aorta, smooth muscle
• UNSPSCコード: 51111800
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: peroxidase conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 1A4, monoclonal
• 形状: lyophilized powder
• 分子量: antigen ~42 kDa
• 化学種の反応性: frog, mouse, hamster, chicken, viper, bovine, crayfish, goat, rat, cat, sheep, guinea pig, lizard, human, rabbit, dog, snail
• テクニック: direct ELISA: suitable; immunoblotting: 2-4 μg/mL using extracts of mouse heart tissues; immunohistochemistry: 5-10 μg/mL using human appendix or tonsil tissue sections
• アイソタイプ: IgG2a
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ACTA2(59)

詳細

詳細

Monoclonal Anti-Actin, α-Smooth Muscle-HRP (mouse IgG2a isotype) is derived from the hybridoma 1A4 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to a sequence of α-smooth muscle actin, conjugated to KLH. Actin is a highly conserved protein, expressed as six isoforms and represents differentiation markers of muscle tissues.

免疫原

a synthetic peptide corresponding to a sequence at the NH2 terminal synthetic decapeptide of α-smooth muscle actin, conjugated to KLH.

アプリケーション

Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody has been used in:

• direct-immunoblotting
• direct-immunohistochemistry
• direct- enzyme linked immunosorbent assay (ELISA)
• direct staining of tissues and cells
• characterization of stromal cell heterogeneity in various organs and distinguish smooth muscle cells from fibroblasts in mixed cultures.

生物化学的／生理学的作用

Actin and Myosin are two major cytoskeletal proteins implicated in cell motility, both constitutively expressed in many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. It has been shown that relative proportions of actin isoforms are diverse in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions.

物理的形状

Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline

調製ノート

Reconstitute in 0.1mL of distilled water.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H315,H317,H319,H334,H412
• 注意書き: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Aquatic Chronic 3 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable