SAB4200366

Anti-IKZF1/IKAROS antibody, Mouse monoclonal

clone E1.14, purified from hybridoma cell culture

• 別名: 抗Hs.54452抗体, 抗IK1抗体, 抗LYF1抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: E1.14, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~55 kDa
• 化学種の反応性: rat, monkey, human, mouse
• 濃度: ~1.0 mg/mL
• テクニック: flow cytometry: suitable; immunocytochemistry: 0.25-0.5 μg/mL using Jurkat cells; indirect immunofluorescence: suitable; western blot: 0.25-0.5 μg/mL using Ramos cells
• アイソタイプ: IgG2b
• UniProtアクセッション番号: Q13422
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... IKZF1(10320)

詳細

詳細

Monoclonal Anti- IKZF1 (Ikaros family zinc finger protein 1)/IKAROS (mouse IgG2b isotype) is derived from the hybridoma E1.14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide located at the C-terminus human IKZF1/IKAROS. The IKAROS gene encodes a family of hemopoietic specific zinc finger transcriptional regulator proteins, which binds to specific DNA target sequences consisting of TGGGAA consensus motif.

免疫原

a peptide located at the C-terminus human IKZF1/IKAROS.

アプリケーション

Monoclonal Anti-IKZF1 (Ikaros family zinc finger protein 1)/IKAROS antibody produced in mouse has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry
• flow cytometry

生物化学的／生理学的作用

Ikaros family zinc finger protein 1 (IKZF1)/IKAROS activity is essential for the generation of B cell precursors. Ikaros is essential at the earliest stage of T cell and B cell specification. In adults, however, lymphoid lineages rely on Ikaros at distinct phases of their development. Mutations or polymorphisms are associated with human B-cell acute lymphoblastic leukemia (B-ALL). Ikaros functions as a tumor suppressor in T-cell acute lymphoblastic leukemia (T-ALL) and B-ALL. Ikaros deletion was detected in patients with blast-phase myeloproliferative neoplasms.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable