S9194

SYBR^® Green JumpStart^™ Taq ReadyMix^™ for High Throughput qPCR

SYBR^® Green qPCR reagent, passive reference dye included

• UNSPSCコード: 41106300
• NACRES: NA.55

特性

• 品質水準: 200
• 形状: liquid
• 使用法: sufficient for 20 reactions; sufficient for 2000 reactions; sufficient for 400 reactions
• 特徴: dNTPs included; hotstart
• 濃度: 1.25 units/reaction (50 μL reaction volume)
• テクニック: qPCR: suitable
• 色: colorless
• 入力: purified DNA
• 適合性: for use with ABI 5700; for use with ABI 7000; for use with ABI 7300; for use with ABI 7700; for use with ABI 7900 Fast; for use with ABI 7900 HT; for use with ABI 7900; for use with ABI StepOne; for use with ABI StepOnePlus
• 検出方法: SYBR^® Green
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

SYBR^® Green JumpStart^™ Taq ReadyMix^™ is a ready-to-use 2X master mix that contains SYBR^® Green I, JumpStart^™ Taq DNA polymerase, 99% pure deoxynucleotides (dNTPs), and reaction buffer making it the ideal solution for performing high-throughput quantitative PCR. SYBR^® Green I dye binds to and detects any dsDNA generated during amplification. The JumpStart^™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

アプリケーション

SYBR^® Green JumpStart^™ Taq ReadyMix^™ for High Throughput qPCR has been used for the amplification and quantification of transcripts to analyze gene expression in 2-step quantitative reverse transcription PCR (RT-qPCR). It has also been used for quantitative polymerase chain reaction (qPCR) of reverse-transcribed cDNA.

特徴および利点

• The master mix allows consistency and reproducibility from one reaction to the next
• Reduced set-up time as compared to manual or wax Hot Start methods
• Reduced primer dimers
• Allows for room temperature set-up; Ideal for high throughput, quantitative PCR application

• SYBR^® Green I dye binds to double-stranded DNA and is ideal for quantifying any DNA sequence. Detection is monitored by measuring the increase in fluorescence throughout cycling.
• JumpStart Taq DNA polymerase prevents amplification of non-specific products while increasing target yield.
• Internal Reference Dye is provided for reaction normalization. Maximum excitation and emission is 586 nm and 605 nm, respectively.
• SYBR Green JumpStart Taq ReadyMix reduces preparation time and the risk of contamination from multiple pipetting steps.

包装

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

その他情報

SYBR Green ReadyMix for High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq and SYBR Green I in an easy-to-use ReadyMix solution that incorporates ROX dye for ABI and other real time instrument applications. The ReadyMix includes a detection fluor, internal standard and reagents for PCR making it the ideal solution for performing high-throughput quantitative PCR.

法的情報

本製品を使用する場合、米国特許番号 5,994,056、6,171,785と、対応する米国外での特許請求のうち、1つ以上が適用されます。本製品の購入について、購入者自身の内部研究のために購入した量を使用する場合に限り、前述の特許請求に基づく訴訟から免責されますが、これは限定的なものであり、譲渡することはできません。他の特許請求(米国特許番号6,814,934に含まれる装置やシステムなど)によって保護されている権利や、あらゆる種類の商業サービス(購入者の研究結果を報酬やその他の商業的事由のために無制限に報告することを含む)を行う権利が、暗示や禁反言によって明示的に譲渡されるわけではありません。本製品は研究目的専用です。Rocheの特許に基づき診断に利用する場合は、別途Rocheからライセンスを受ける必要があります。ライセンス購入に関する詳細な情報については、ライセンス管理者(Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA)にお問い合わせ下さい。

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies