S6197

抗STIM1 (C-末端) ウサギ宿主抗体

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗GOK抗体, 抗間質相互作用分子1抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 90 kDa
• 化学種の反応性: human, rat, mouse
• 濃度: ~1 mg/mL
• テクニック: immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells.; indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells.; western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells.
• UniProtアクセッション番号: Q13586
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... STIM1(6786) ; mouse ... Stim1(20866) ; rat ... Stim1(117086)

詳細

詳細

Stromal interaction molecule 1 (STIM1) is a conserved single-pass transmembrane protein. STIM1 is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 localizes predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca²⁺ levels.

免疫原

ヒトSTIM1のアミノ酸657~683に相当する合成ペプチド (N末端システイン付加) のKLH結合体。相当する配列は、ラット、マウス、ウシ、サル、イヌと同一です。

アプリケーション

Anti-STIM1 (C-terminal) antibody produced in rabbit has been used in:

• western blot
• immunoprecipitation
• immunofluorescence
• immunohistochemistry

生物化学的／生理学的作用

Stromal interaction molecule 1 (STIM1) is required for the activation of store-operated Ca²⁺ influx. STIM1 undergoes rapid oligomerization and redistributes into discrete spots (punctae) that move towards and accumulate in the cell periphery, possibly to activate Orai1 that is located in the plasma membrane. Overexpression of STIM1 and Orai1 together markedly increases the calcium release-activated channels (CRAC) current (I-CRAC). Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex plays a major role in the activation of store-operated calcium (SOC) channel in response to internal Ca²⁺ store depletion.

物理的形状

0.01 M PBS溶液 (pH 7.4, 15 mM アジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)