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由来生物
mouse
結合体
unconjugated
抗体製品の状態
purified from hybridoma cell culture
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
SIR11, monoclonal
形状
buffered aqueous solution
分子量
antigen ~105 kDa
化学種の反応性
mouse
濃度
~2 mg/mL
テクニック
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of C-2 cells
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Sirt1(93759)
詳細
Monoclonal Anti-Sirt1 (Sir2) (mouse IgG1 isotype) is derived from the hybridoma SIR11 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids at the C-terminus of mouse Sir2 with C-terminal added lysine. Sirt proteins are a family of proteins that are found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity. Sirtuin 1 (SIRT1) protein, a mammalian homolog of yeast Sir2, is a NAD-dependent histone deacetylase.
特異性
Monoclonal Anti-Sirt1 (Sir2α) antibody is specific for Sir2α in mice.
アプリケーション
Anti-Sirt1 antibody, Mouse monoclonal has been used in:
- western blot analysis
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemical methods
Monoclonal Anti-Sirt1 antibody is suitable for use in western blot (1-2 μg/mL using total cell extract of C-2 cells), immunoblot (approx. 105 kDa), microarray and indirect ELISA.
生物化学的／生理学的作用
SIRT1 is a highly conserved sirtuin family deactelyase that modulates heart metabolism . SIRT1 has also been implicated in senescence and aging . Thus, SIRT1 may be used as a target for treating age-related diseases.
Sirt1 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes O-acetyl-ADP-ribose. Through histone deacetylation, Sirt1 may silence chromatin. It appears that Sirt1 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction. Sirtuin 1 (SIRT1) plays an important role in neuronal proliferation and vascular cells. It also plays an athero-protective role. Mutations in the SIRT1 gene affects the metabolism, cholesterol and fat deposition. SIRT1 has the ability to intermediate the insulin resistance through an inflammatory process, reactive oxygen species, gluconeogenesis and adiponectin level.
物理的形状
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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製品番号
詳細
価格
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
S5196-VAR:
S5196-BULK:
S5196-200UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Yu Wang et al.
Pharmacology & therapeutics, 136(3), 305-318 (2012-09-04)
Sirtuin 1 (SIRT1), the mammalian ortholog of yeast Sir2p, is a highly conserved NAD(+)-dependent protein deacetylase that has emerged as a key cardiometabolic regulator. During the past decade, Sir2p has been the focus of intense investigations and discussion because it
Hepatic NAD+ deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing
Zhou CC, et al.
British Journal of Pharmacology, 173(15), 2352- 2368 (2016)
Sirt1 improves healthy ageing and protects from metabolic syndrome-associated cancer
Herranz D, et al.
Nature Communications, 1(28), 3-3 (2010)
Jing Lu et al.
EMBO reports, 18(5), 841-857 (2017-04-02)
SIRT1, the most conserved mammalian NAD+-dependent protein deacetylase, is an important metabolic regulator. However, the mechanisms by which SIRT1 is regulated in vivo remain unclear. Here, we report that phosphorylation modification of T522 on SIRT1 is crucial for tissue-specific regulation
Sivaram Pillarisetti
Recent patents on cardiovascular drug discovery, 3(3), 156-164 (2008-11-11)
Sirt1 (member of the sirtuin family) is a nicotinamide adenosine dinucleotide (NAD)-dependent deacetylase that removes acetyl groups from various proteins. A wide variety of proteins are Sirt1 substrates; the list includes many transcription factors and cofactors. Deacetylation of these factors
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