RTN9604

GenElute^™ 96ウェル トータルRNA 精製キット

4 x 96 well plate, sufficient for 4 96-well plate purifications

• 別名: High Throughput Purification, RNA Purification
• UNSPSCコード: 41105501
• NACRES: NA.52

特性

• 使用法: sufficient for 4 96-well plate purifications
• 品質: 4 x 96 well plate
• 環境により配慮した代替製品の特徴: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• テクニック: RNA purification: suitable
• 環境により配慮した代替製品カテゴリ: Aligned,
• 保管温度: 15-25°C

詳細

詳細

シグマのGen Elute 96 Well Total RNA Purification Kitは、細胞や組織からtotal RNAを簡便に分離することができます。シリカメンブレン法のメリットを利用しているため、フェノールやクロロホルムなど危険な有機化合物を使う必要がありません。

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

アプリケーション

The purified total RNA is ready for use in any enzymatic downstream application.

特徴および利点

• ハイスループットなフォーマットにより、RNAの迅速な精製が可能です。
• 真空下でも遠心分離にも使用できます。
• 自動化システムでもマニュアル操作でも使用できます。
• RNAは、あらゆる種類の酵素反応に使用できます。
• 独特のプレートデザインにより、交差汚染のリスクが低く抑えられています。

原理

Sigma′s GenElute 96 Well Total RNA Purification Kit provides a simple and convenient way to isolate total RNA from cells or tissue. The cells or tissue are lysed in a solution containing large amounts of chaotropic salt which immediately inactivates RNases that are present in almost all biological materials. The addition of a Wash Buffer provides the necessary binding conditions for the RNA to be adsorbed to the silica membrane. The contaminating DNA which may be bound to the silica membrane is removed by directly applying RNase-free DNase. The remaining salts, proteins, and other cellular debris are removed by additional washing steps. The final step is to elute the purified RNA in the RNAase-free water that is supplied in the kit.

その他情報

Application notes:
Method of purification:Silica-membrane technology
Compatible with Manual or automated, vacuum or centrifugation
Efficient recovery of > 200 nt
Typical yield20 μg (2 x 106 HeLa cells),
20 μg (20 mg mouse liver)
Typical concentration50–200 ng/μL
Elution volume50–130 μL
Preparation time70 min/plate
Binding capacity100 μg
RNA ratio28S/18S approx. 2.1
A₂₆₀/A₂₈₀1.90–2.10
Typical RIN (RNA > 9 (cells)
integrity number)≥ 7 (tissue)
Sufficient for:
< 107 cultured cells (centrifugation)
< 30 mg tissue (centrifugation)
saliva

Related products:
Prod #Related prod #
RTN9604RTN9602
RTN9602RTN9604

法的情報

GenEluteはシグマアルドリッチの登録商標です。

GenElute is a trademark of Sigma-Aldrich Co. LLC

キットの構成要素のみ

• RNA Lysis Solution 125 mL
• RNA Wash Buffer 1 360 mL
• RNA Wash Buffer 2, Concentrate 2 x 90
• RNA Wash Buffer 3, Concentrate 2 x 54
• Reaction Buffer for rDNase 4 x 20
• rDNase, RNase-free 8 vial(s)
• RNase-free Water 2 x 54
RNA Binding Plates, blue ring 4 ea
Collection Tubes, 1.5 mL 16 ea
Wash Plates 4 ea
Square-well Blocks 6 ea
Elution Plate, U-Bottom 4 ea
Round-well Blocks Low 4 ea

すべて表示 (13)

安全性情報

• ピクトグラム: GHS08,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H302 + H332,H314,H317,H334,H412
• 注意書き: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1
• 補足的ハザード: EUH032
• 保管分類コード: 8A - Combustible corrosive hazardous materials
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable