R3025

抗Rho-GDI抗体 ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL

• 別名: Anti-GDIA1, Anti-HEL-S-47e, Anti-NPHS8, Anti-RHOGDI, Anti-RHOGDI-1
• MDL番号: MFCD01634148
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~28 kDa
• 化学種の反応性: mouse, bovine, human
• 濃度: ~1 mg/mL
• テクニック: immunoprecipitation (IP): 4 μg using 500 μg of Jurkat RIPA lysate; western blot: 0.5-1.0 μg/mL using RIPA lysates from human HL-60, HeLa, Hs294T, Jurkat and murine 3T3/NIH cells and mouse 3T3/A31
• UniProtアクセッション番号: P52565
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ARHGDIA(396) ; mouse ... Arhgdia(192662)

詳細

詳細

Rho guanine nucleotide dissociation inhibitor I (RhoGDI), also known as Rho GDP dissociation inhibitor α (ARHGDIA) is encoded by the gene mapped to human chromosome 17q25.3-25-3. RhoGDI is the member of Rho/Rac protein family. The encoded protein consists of 204 amino acids and has a molecular mass of 23,421,000 Mr. In mammals, three RhoGDI isoforms exists: RhoGDIα, RhoGDIβ and RhoGDIγ. RhoGDIα is widely expressed. RhoGDIβ is mainly expressed in hematopoietic cells and RhoGDIγ is present in the brain, lung, kidney and testis.

Rho proteins are basically described as guanine nucleotide-binding proteins (GNBPs). RhoGDI (Rho guanine nucleotide dissociation inhibitor)/ARHGDIA (Rho guanine nucleotide dissociation inhibitor) has three subtypes namely- RhoGDIα (Rho GDP dissociation inhibitor α), RhoGDIβ (Rho GDP dissociation inhibitor β) and RhoGDIγ (Rho GDP dissociation inhibitor γ). RhoGDIα is expressed everywhere. It is located on human chromosome 17q25.3. Its protein has 204 amino acids and belongs to the Rho/Rac family.

特異性

Rho-GDIを認識し、D4-GDIと交差反応性があります。

免疫原

完全長ヒトRho-GDIに相当するGST融合タンパク質。

アプリケーション

Anti-Rho-GDI antibody produced in rabbit has been used in immunoblotting.

Anti-Rho-GDI has been used in western blotting.

生物化学的／生理学的作用

Rho proteins control the actin and microtubule cytoskeleton. RhoGDIα is necessary for the spatial and temporal modulation of RhoGNBPs (guanine nucleotide-binding proteins). In HCC (hepatocellular carcinoma) patients, absence of ARHGDIA (Rho guanine nucleotide dissociation inhibitor) affects the prognosis and induces invasion and metastasis of HCC cells.

物理的形状

0.1Mトリス-グリシン溶液(pH 7.4, 0.15M NaCl, 0.05%アジ化ナトリウム含有)。タンパク質濃度は約1 mg/mLです。

調製ノート

プロテインAで精製済み。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable