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詳細
β-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for β-lactam antibiotics. This enzyme is present in Gram-negative bacteria.
アプリケーション
β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder.
β-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures.
生物化学的/生理学的作用
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.
単位の定義
1ユニットは、pH 7.0、25°C、1分間に、1.0 μmolのベンジルペニシリンを加水分解する酵素量です。この国際単位(ベンジルペニシリンを基質として使用)は、600 Levyユニットまたは75 Pollockユニットとほぼ同等です。
物理的形状
凍結乾燥品(リン酸ナトリウムバッファ-塩を含有)
アナリシスノート
タンパク質はBiuret法で測定。
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Resp. Sens. 1 - Skin Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
試験成績書(COA)
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Protein-protein interactions monitored in mammalian cells via complementation of beta-lactamase enzyme fragments
Proceedings of the National Academy of Sciences of the USA, 99(6), 3469-3474 (2002)
Persistent bacteremia in rabbit fetuses despite maternal antibiotic therapy in a novel intrauterine-infection model
Antimicrobial Agents and Chemotherapy, 47(7), 2125-2130 (2003)
Past and present perspectives on beta$-lactamases
Antimicrobial Agents and Chemotherapy, 62(10) (2018)
Genetics, 181(4), 1521-1533 (2009-02-05)
We present a new hypothesis for the selective pressures responsible for maintaining natural competence and transformation. Our hypothesis is based in part on the observation that in Bacillus subtilis, where transformation is widespread, competence is associated with periods of nongrowth
Bioorganic & medicinal chemistry letters, 19(4), 1261-1263 (2009-01-27)
An efficient synthesis of a 5-fluorouracil-cephalosporin prodrug is described for use against colorectal and other cancers in antibody and gene-directed therapies. The compound shows stability in aqueous media until specifically activated by beta-lactamase (betaL). The kinetic parameters of the 5-fluorouracil-cephalosporin
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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