L6170

β-ラクタマーゼ 緑膿菌由来

recombinant, expressed in E. coli

• 別名: Cephalosprinase, カルバペネマーゼ
• CAS番号: 9073-60-3
• Enzyme Commission number: 3.5.2.6 (BRENDA, IUBMB)
• EC Number: 232-970-8
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• リコンビナント: expressed in E. coli
• 品質水準: 200
• 形状: powder
• 比活性: ≥20 U/mg (with cephalosporin C); ≥400 U/mg (with benzylpenicilin)
• 保管温度: 2-8°C

詳細

詳細

β-Lactamase produced by bacteria is closely related to the penicillin binding proteins. β-Lactamase hydrolyzes β-lactum antibiotics and is the prime cause of resistance development by bacteria. There are four subclasses of β-Lactamases. Classes A, C and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes with zinc ion at their active site for β-lactam hydrolysis. Mutations in the β-lactamases has resulted in the generation of extended-spectrum β-lactamases (ESBLs). As close to 900 types of β-Lactamases are produced by microbes.

アプリケーション

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product L6170 is recombinantly produced based on the sequence of the enzyme from Pseudomonas aeruginosa, and is expressed in E. coli.

β-Lactamase has been used in coumarin-cephalosporin-fluorescein-acetoxymethyl (CCF4)- β-Lactamase assay in dendritic cells and in the hydrolysis of meropenem (H-Mer) to generate 18O-labeled H-Mer.

生物化学的／生理学的作用

他の基質の一般的解析:セフトリアキソン 2～4 unit/mg、セファゾリン2～4 unit/mg、セフタジジム1.5～3 unit/mg、セホキシチン0.35～0.7 unit/mg、セフェピム1.2～2.4 unit/mg、セフロキシム1.5～3 unit/mg、セフォタキシム1～2 unit/mg、オキサシリン4～8 unit/mg、イミペネム1.2～2.4 unit/mg、メロペネム3～6 unit/mg

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring. Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg

単位の定義

One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in 50 mM phosphate buffer, pH 7.0 containing 10 μM zinc chloride at 25 ºC.

物理的形状

凍結乾燥粉末 (塩化ナトリウムおよびン酸カリウム含有)

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable