P4306

血小板由来成長因子-BB ヒト

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 別名: PDGF-BB
• MDL番号: MFCD00213023
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• product name: 血小板由来成長因子-BB ヒト, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in E. coli
• アッセイ: ≥97% (SDS-PAGE)
• 形状: lyophilized powder
• 有効性: 1.0-3.0 ng/mL EC₅₀
• 品質: endotoxin tested
• 分子量: 24.3 kDa
• 包装: pkg of 10 μg
• 保管条件: avoid repeated freeze/thaw cycles
• テクニック: cell culture | mammalian: suitable
• 不純物: ≤1 EU/μg
• UniProtアクセッション番号: P01127
• 保管温度: −20°C
• 遺伝子情報: human ... PDGFB(5155)

詳細

詳細

Platelet-Derived Growth Factor (PDGF), a cationic polypeptide, is an important serum mitogen predominantly found in cells of mesenchymal origin. PDGF is mapped to human chromosome 7p22.3 and is expressed in smooth muscle cells, endothelial cells and macrophages. PDGF exists as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

アプリケーション

Platelet-Derived Growth Factor-BB human has been used in induction of DNA synthesis and proliferation in low-concentration serum.

生物化学的／生理学的作用

Platelet-derived growth factor (PDGF) is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF elicits multifunctional actions in a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. PDGF-BB isomer is highly expressed in tumor, mediating oncogenic pericyte recruitment and is is a key regulator of angiogenesis. PDGF-BB controls bone homeostasis, repair and regeneration. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins and modulates LDL receptor binding.

その他情報

ヒトPDGFのB鎖からなる二量体

物理的形状

0.2 μmフィルタ-で濾過した溶液の凍結乾燥品

アナリシスノート

生物活性は、BALB/c 3T3細胞のthymidine取り込みに対する用量依存的な促進活性として測定されています。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)