P2092

モノクロナール抗p27^Kip1 マウス宿主抗体

clone DCS-72, ascites fluid

• MDL番号: MFCD00284080
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ICC; IP; WB
• クローン: DCS-72, monoclonal
• 化学種の反応性: human, rat, monkey, canine, mouse
• citations: 12
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 1:200 using extract of cultured mouse fibroblast line NIH3T3

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: DCS-72, monoclonal
• 分子量: antigen 27 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: human, rat, monkey, canine, mouse
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 1:200 using extract of cultured mouse fibroblast line NIH3T3
• アイソタイプ: IgG1
• UniProtアクセッション番号: P46527
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CDKN1B(1027) ; mouse ... Cdkn1b(12576) ; rat ... Cdkn1b(83571)

詳細

詳細

Monoclonal Anti p27Kip1 (mouse IgG1 isotype) is derived from the DCS-72 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant p27Kip1 protein of rodent origin.

特異性

イムノブロッティングの場合、サンプルによっては高分子量および低分子量の分子のバンドがみられる可能性もあります。これらは、p27^Kip1がポリユビキチン化されているか、あるいは切断産物であると考えられます。

免疫原

齧歯類のリコンビナントp27^Kip1タンパク質。

アプリケーション

Monoclonal Anti-p27Kip1 antibody produced in mouse has been used in immunoblotting, immunofluorescence, immunohistochemistry, immunoprecipitation and immunocytochemistry.

生物化学的／生理学的作用

p27, the product of the kinase inhibition protein1 (kip1) gene, is an inducible inhibitor of cyclin dependent kinase activity. p27Kip1 interacts strongly with D-type cyclins complexed with CDK4 and more weakly with cyclin E and CDK2 complexes. Regulation by p27Kip1 may be an essential step in the pathway that links mitogenic signals to cell cycle progression and may be a key molecular event in the physiological process of cell cycle commitment or passage through the restriction point. Thus, the amount of p27Kip1 increases in quiescent cells and rapidly decreases after stimulation with specific mitogens. Constitutive expression of p27Kip1 in cultured cells causes cell cycle arrest in G1.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable