N1665

5′-ヌクレオチダーゼ ヒト

recombinant, expressed in CHO cells, vial of 6-12 μg

• 別名: CD73, NT5E, ecto-5′-nucleotidase
• CAS番号: 9027-73-0
• Enzyme Commission number: 3.1.3.5 (BRENDA, IUBMB)
• MDL番号: MFCD00130311
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• リコンビナント: expressed in CHO cells
• 品質水準: 200
• 詳細: contains a C-terminal 6-His tag
• アッセイ: ≥90% (SDS-PAGE)
• フォーム: solution
• enzyme activity: ≥15 units/mg protein
• 分子量: ~61 kDa by SDS-PAGE (reducing)
• 包装: vial of 6-12 μg
• 不純物: ≤1.0 EU/μg endotoxin
• NCBIアクセッション番号: NP_002517
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... NT5E(4907)

詳細

詳細

5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.

生物化学的／生理学的作用

5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.

5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.

単位の定義

1 unitは、pH 9.0、37°C、1分間に、1.0 μmoleの無機リンをアデノシン5'-一リン酸から加水分解する量です。

物理的形状

Supplied as a solution containing Tris, NaCl, CaCl_2, and 20% glycerol.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable