MAK555

Lysyl Oxidase (LOX) Assay Kit

sufficient for 500 fluorometric tests

• UNSPSCコード: 41116104
• NACRES: NA.84

特性

• 検出方法: fluorometric
• 関連疾患: dermatological diseases; orthopedic diseases; pulmonary disorders
• 保管温度: −20°C

詳細

詳細

Lysyl oxidase (LOX) is an extracellular enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. These aldehydes are highly reactive and undergo spontaneous chemical reactions with other lysyl oxidasederived aldehyde residues, or with unmodified lysine residues. This results in cross-linking collagen and elastin which is essential for stabilization of collagen fibrils and for the integrity and elasticity of mature elastin. Lysyl oxidase activity in biological samples is traditionally and most reliably assessed by tritium release end-point assays using radiolabeled collagen or elastin substrates involving laborious vacuum distillation of the released tritiated water. ​

The Lysyl Oxidase Assay Kit offers a sensitive fluorescent assay to measure LOX activity using a LOX substrate that releases hydrogen peroxide upon oxidation. The amount of hydrogen peroxide released by the LOX oxidation is detected using HRP substrate in the HRP-coupled reactions. This allows the detection of sub ng/mL lysyl oxidase. This method eliminates the interference that occurs in some biological samples and can be readily used to detect lysyl oxidase activity in cell culture samples.

その他情報

Please note that the kit does not include the lysyl oxidase enzyme.

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H302,H317,H334,H360FD
• 注意書き: P202 - P261 - P280 - P301 + P312 - P302 + P352 - P308 + P313
• 危険有害性の分類: Acute Tox. 4 Oral - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• 引火点(°F): 188.6 °F - closed cup
• 引火点(℃): 87 °C - closed cup