M8177

モノクロナール抗p38MAPキナーゼ, 活性化 (二リン酸化型p38) マウス宿主抗体

clone P38-TY, purified from hybridoma cell culture, buffered aqueous solution

• 別名: 抗MAPキナーゼ, p38, 活性化型抗体
• MDL番号: MFCD00803556
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: P38-TY, monoclonal
• 形状: buffered aqueous solution
• 化学種の反応性: rat, human, mouse
• 濃度: ~2 mg/mL
• テクニック: immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1 μg/mL using whole cell extract from a rat fibroblast cell line, Rat1, activated with sorbitol
• アイソタイプ: IgG2a
• UniProtアクセッション番号: Q16539
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MAPK14(1432) ; mouse ... Mapk14(26416) ; rat ... Mapk14(81649)

詳細

詳細

Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) (mouse IgG2a isotype) is derived from the P38-TY hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The p38 mitogen-activated protein kinases (MAPK) comprises the stress-activated protein kinase (SAPK) 2b, SAPK3 and SAPK4 (also termed p38β-δ). All these kinases contain a glycine residue in their TXY(Thr−Xaa−Tyr motif) activation motif.

特異性

二リン酸化された活性型のp38 MAPキナ-ゼおよび類縁のアイソフォ-ムと特異的に反応します。p38 MAPキナ-ゼの非リン酸化型および一リン酸化型分子をはじめ、非リン酸化型, 一リン酸化型, 二リン酸化型のJNKやERK-MAPキナ-ゼは認識されません。抗体が認識するエピト-プには、p38 MAPキナ-ゼの調節部位内のリン酸化スレオニンおよびチロシン(Thr180-Gly181-Tyr182)が含まれています。

免疫原

Synthetic peptide HTDDEMpTGpYVATR corresponding to the phosphorylated form of p38 MAP kinase activation loop, coupled to KLH.

アプリケーション

Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody produced in mouse may be used in immunoblotting, enzyme linked immunosorbent assay (ELISA), immunostaining, western blotting, immunohistochemistry and in immunocytochemistry.

生物化学的／生理学的作用

The p38 mitogen-activated protein kinases (MAPK) are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs, and is glutamic acid for extracellular-signal regulated kinase (ERK) (TEY), proline for c-Jun N-terminal kinases (JNK) and glycine for p38 MAPK. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. ERK generally transmits signals leading to cell proliferation, p38 MAPK and JNK are both mostly stress-responsive kinases3 and have been implicated in cell death in several cellular systems. Antibodies reacting specifically with activated p38 MAPK are useful tools in the study of the intracellular location of p38 MAPK enzymes, and in sorting out the signal transduction pathways of the MAPK superfamily.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

調製ノート

バイオリアクタ-で増殖させたハイブリド-マの培養上清から精製。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable