C7492

Monoclonal Anti-Calreticulin antibody produced in mouse

clone TO-11, tissue culture supernatant, buffered aqueous solution

• 別名: Calreticulin Antibody Flow Cytometry, Anti-Autoantigen Ro
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: tissue culture supernatant
• 抗体製品タイプ: primary antibodies
• クローン: TO-11, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~50 kDa
• 化学種の反応性: human
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 1:50-1:100 using total cell extract of HeLa cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P27797
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CALR(811)

詳細

詳細

Monoclonal Anti-Calreticulin (mouse IgG1 isotype) is derived from the hybridoma TO-11 produced by the fusion of mouse myeloma cells (P3-X63-AG8.653 cells) and splenocytes from BALB/c mice.

Monoclonal Anti-Calreticulin (mouse IgG1 isotype) is derived from the hybridoma TO-11 produced by the fusion of mouse myeloma cells (P3-X63-AG8.653 cells) and splenocytes from BALB/c mice. Calreticulin is a molecular chaperones in endoplasmic reticulum lumen. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins, and misfolded proteins. Calreticulin is a protein binding Ca²⁺ ions. It is encoded by the CALR gene in humans.

アプリケーション

Monoclonal Anti-Calreticulin antibody produced in mouse is suitable for the following applications:

• Flow cytometry
• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Western blotting

生物化学的／生理学的作用

Calreticulin and Its variants causes differential effects on thrombopoiesis. Somatic mutations of calreticulin (CALR) gene causes thrombocythemia (ET) and primary myelofibrosis (PMF).

It is believed to play a critical role in quality control processes during protein synthesis and folding. Increased expression of calreticulin increases Ca²⁺ storage capacity of the ER. It also appears to modulate store-operated Ca²⁺ -influx, and to alter Ca²⁺ transport by the sarcoplasmic/ER Ca²⁺ -ATPase (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. In contrast, calreticulin-deficient cells show increased resistance to apoptosis. Calreticulin gene disruption leads toimpairment of cardiac development. Loss of calreticulin function favors ubiquitin-proteosome activity. It plays a critical role in Ca²⁺ homeostasis.

物理的形状

Culture supernatant solution containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)