C4606

Anti-Calreticulin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL番号: MFCD01092536
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 50 kDa
• 交差性: human, canine
• テクニック: immunoprecipitation (IP): suitable using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line; indirect immunofluorescence: 1:100 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line; microarray: suitable; western blot: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line
• UniProtアクセッション番号: P27797
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CALR(811)

詳細

詳細

The gene for calreticulin (CALR) is located on the human chromosome 19p13.13. The encoded protein is a predominantly conserved calcium-binding chaperone, which is localized primarily to the lumen of the endoplasmic reticulum (ER). Calreticulin comprises three domains, a globular N-domain, a proline-rich P-domain, and a highly acidic C-domain.

特異性

Anti-Calreticulin recognizes human and dog calreticulin (55-60 kDa).

免疫原

ヒトカルレティキュリンのC末端(アミノ酸401-417)に相当する合成ペプチド。

アプリケーション

Anti-Calreticulin antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunocytochemistry{91
• immunoprecipitation

生物化学的／生理学的作用

Calreticulin facilitates transient interaction with newly synthesized cellular and extracellular proteins for folding and assembling in the endoplasmic reticulum (ER) before its localization to the cytosol or cell surface. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins and misfolded proteins. It is believed to play a critical role in quality control processes during protein synthesis and folding and calcium (Ca²⁺) homeostasis. Increased expression of calreticulin increases the Ca²⁺ storage capacity of the ER. It also appears to modulate store-operated Ca²⁺-influx and to alter Ca²⁺ transport by the sarcoplasmic/ER Ca²⁺-adenosine triphosphatase (ATPase) (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. However, increased resistance to apoptosis has been observed in calreticulin-deficient cells.

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

保管および安定性

Store at -20 °C. For continuous use, the product maybe stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at-20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded ifnot used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable