M6065

モノクロナール抗Microphthalmia マウス宿主抗体

clone C5, purified immunoglobulin

• 別名: 抗Mi抗体
• MDL番号: MFCD00802742
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: IHC (p); IP; WB
• クローン: C5, monoclonal
• 化学種の反応性: mouse, rat, human
• citations: 5
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable using 2 μg/mg protein lysate; western blot: 1:500 using 10 μg of Mouse brain lysates

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: C5, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 52-56 kDa
• 化学種の反応性: mouse, rat, human
• 濃度: 0.5-1.0 mg/mL
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable using 2 μg/mg protein lysate; western blot: 1:500 using 10 μg of Mouse brain lysates
• アイソタイプ: IgG1
• UniProtアクセッション番号: O75030
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MITF(4286) ; mouse ... Mitf(17342) ; rat ... Mitf(25094)

詳細

詳細

Microphthalmia (Mi in mouse or MITF in human) is a basic helix-loop-helix-leucine zipper (BHLH–ZIP) transcription factor involved in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium, i.e. cells responsible for hair, skin, and eye color. It activates the expression of the melanocyte specific genes tyrosinase and TRP1 (tyrosinase-related protein 1) by binding as a homo- or heterodimer to a symmetrical DNA sequence (E box) (5′-CATGTG-3′) located within the M box found in their promoters. Microphthalmia also appears to be involved in the differentiation of mast cells, osteoclasts, basophils and natural killer cells.
Microphthalmia is expressed in a limited number of cell types including heart, mast cells, osteoclast precursors, and melanocytes. There are a number of different isoforms of microphthalmia resulting from alternative splicing and alternative promotors. These isoforms differ at their amino-termini and in their expression patterns.

Mouse monoclonal clone C5 anti-Microphthalmia antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia from human, mouse or rat.

免疫原

ヒト小眼球症タンパク質のN末端フラグメント。

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal clone C5 anti-Microphthalmia antibody is used to tag serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia for detection and quantitation by techniques such as immunoblotting (doublet of 52-56 kDa), immunoprecipitation, immunohistochemistry on formalin-fixed paraffin embedded or frozen tissue sections, and gel shift. It is used as a probe to determine the roles of microphthalmia in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium.

物理的形状

Solution in phosphate buffered saline containing 0.08% sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable