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Merck

GE17-5446-02

Ficoll®-Paque PREMIUM 1.084

Cytiva 17-5446-02, pack of 6 × 100 mL

別名:

Ficoll PREMIUM

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About This Item

UNSPSCコード:
12352201
NACRES:
NA.25

形状

liquid

包装

pack of 6 × 100 mL

メーカー/製品名

Cytiva 17-5446-02

Colorless to slightly yellow

pH範囲

5.5-7.5

詳細

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.

アプリケーション

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

特徴および利点

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2003.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Ficoll is a registered trademark of Cytiva

保管分類コード

12 - Non Combustible Liquids


試験成績書(COA)

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Ficoll&#174;400

Sigma-Aldrich

F9378

Ficoll®400

資料

Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

This page shows methods to remove Percoll from a sample after centrifugation.

This page shows How to fractionate and analyze gradients of Percoll

すべて表示

プロトコル

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

How to use percoll to prepare a gradient

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