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形状
liquid
包装
pack of 6 × 100 mL
メーカー/製品名
Cytiva 17-5446-02
色
Colorless to slightly yellow
pH範囲
5.5-7.5
詳細
Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.
アプリケーション
Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.
Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).
特徴および利点
- Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
- Manufactured under a Quality Management System certified to ISO 13485:2003.
- Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
- Cytotoxicity grade 0.
- A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.
保管および安定性
アナリシスノート
法的情報
保管分類コード
12 - Non Combustible Liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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資料
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
This page shows methods to remove Percoll from a sample after centrifugation.
This page shows How to fractionate and analyze gradients of Percoll
プロトコル
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
How to use percoll to prepare a gradient
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