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メーカー／製品名
Novagen®
保管条件
OK to freeze
保管温度
10-30°C
詳細
The BCA protein assay is a simple and reliable protein quantification method.
The BCA protein assay is based on a biuretreaction, which is the reduction of Cu2+ toCu+ by proteins in an alkaline solution withconcentration-dependent detection of themonovalent copper ions. Bicinchoninic acidis a chromogenic reagent that chelates thereduced copper, producing a purple complexwith strong absorbance at 562 nm (Smith1985, Wiechelman 1988). This assay can beused to quantify protein concentration with awide variety of samples and can be performedin minutes.
The Novagen® BCA Protein Assay Kit canbe used to determine protein concentrationin the range of 20-2000 µg/ml in either astandard assay or microassay configuration.Kit components are sufficient to complete500 standard-size reactions (50 µl proteinsample plus 1 ml reagent) or 2500 micro-scalereactions (25 µl protein sample plus 200 µlreagent). A BSA standard (2 mg/ml) is providedfor convenient and reliable preparation ofstandard curves.
This assay is robust and can be performedin the presence of many compounds. Somereagents, including chelating agents, strongacids or bases, and reducing agents, interferewith the reduction and chelating reactions onwhich this assay depends (Brown 1989). TheBCA assay is compatible with the followingNovagen protein extraction and lysis reagents:BugBuster Protein Extraction Reagent,PopCulture Reagent, CytoBuster ProteinExtraction Reagent, Reportasol ExtractionBuffer, and Insect PopCulture Reagent. Optionsfor the removal or dilution of interferingsubstances are described in the kit literature.
The Novagen® BCA Protein Assay Kit canbe used to determine protein concentrationin the range of 20-2000 µg/ml in either astandard assay or microassay configuration.Kit components are sufficient to complete500 standard-size reactions (50 µl proteinsample plus 1 ml reagent) or 2500 micro-scalereactions (25 µl protein sample plus 200 µlreagent). A BSA standard (2 mg/ml) is providedfor convenient and reliable preparation ofstandard curves.
This assay is robust and can be performedin the presence of many compounds. Somereagents, including chelating agents, strongacids or bases, and reducing agents, interferewith the reduction and chelating reactions onwhich this assay depends (Brown 1989). TheBCA assay is compatible with the followingNovagen protein extraction and lysis reagents:BugBuster Protein Extraction Reagent,PopCulture Reagent, CytoBuster ProteinExtraction Reagent, Reportasol ExtractionBuffer, and Insect PopCulture Reagent. Optionsfor the removal or dilution of interferingsubstances are described in the kit literature.
構成
•500 mlBCA Solution
•15 ml4% Cupric Sulfate
•3 x 1 mlBSA Standard, 2 mg/ml
•15 ml4% Cupric Sulfate
•3 x 1 mlBSA Standard, 2 mg/ml
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
その他情報
Smith, P.K., et al. 1985. Anal. Biochem.150, 76.
Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.
Wiechelman, K., et al. 1988. Anal. Biochem.175, 231.
Brown, R., et al. 1989. Anal. Biochem.180, 136.
Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.
Wiechelman, K., et al. 1988. Anal. Biochem.175, 231.
Brown, R., et al. 1989. Anal. Biochem.180, 136.
法的情報
Use of the BCA protein assay is permitted for research purposes only.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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消防法
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Jan Code
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