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About This Item
結合体:
unconjugated
application:
ICC
IF
IP
IF
IP
クローン:
M3A5, monoclonal
化学種の反応性:
monkey, human, chicken, goose, rabbit, canine, bovine, kangaroo rat, rat, hamster
citations:
25
テクニック:
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:20 using cultured Chinese hamster ovary (CHO) cells
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:20 using cultured Chinese hamster ovary (CHO) cells
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由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
M3A5, monoclonal
含みます
15 mM sodium azide
化学種の反応性
monkey, human, chicken, goose, rabbit, canine, bovine, kangaroo rat, rat, hamster
テクニック
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:20 using cultured Chinese hamster ovary (CHO) cells
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... COPB1(1315)
rat ... Copb1(114023)
詳細
Monoclonal Anti- β-COP (mouse IgG1 isotype) is derived from the M3A5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. COPs (coatomer proteins) have a molar mass of 110 kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles.
The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins.
特異性
抗体は、多くの組織培養系に見いだされるβ-COP(110 kDa)および脳微小管結合タンパク質のダブレット(MAP2, 270-300 kDa)に共通のエピト-プを認識します。抗体は、ニワトリ脳神経細胞の細胞突起や細胞体をはじめ、さまざまな生物種由来組織中の非神経細胞の核周囲にみられる網状構造(ゴルジ複合体の周辺や細胞質に散在するコ-トマ-集団)を染色します。エネルギ-状態に影響を与える因子や、ゴルジ複合体の破壊、細胞内のGタンパク質または低分子量GTP結合タンパク質の活性の変化を引き起こすさまざまな物質についての研究や、β-COPの細胞内局在性に対する効果を研究するために利用されています。本抗体は、タンパク質の免疫アフィニティ-精製に使用することができます。
免疫原
ガチョウ脳由来微小管結合タンパク質。
アプリケーション
Monoclonal Anti-β-COP antibody produced in mouse has been used:
- for the localization of β-COP using immunoprecipitation
- in immunocytochemistry
- in immunoblotting
- with other antibodies to Golgi proteins to study the role and relationships of this protein in the cell
生物化学的／生理学的作用
COPs (coatomer proteins) are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
G2279-VAR:
G2279-.2ML:
G2279-100UL:
G2279-.5ML:
G2279-BULK:
M A Stamnes et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(17), 8011-8015 (1995-08-15)
We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain
D Kondo et al.
Journal of the American Society of Nephrology : JASN, 11(5), 803-813 (2000-04-20)
Agene encoding olfactomedin-related glycoprotein was isolated from rat glomerulus despite its prior identification as a neuron-specific gene. The mRNA expression was remarkably intense in renal glomerulus and brain and faint in the lung and eye among rat systemic organs. Although
L Orci et al.
Nature, 362(6421), 648-652 (1993-04-15)
Do the coats on vesicles budded from the Golgi apparatus actually cause the budding, or do they simply coat buds (Fig. 1)? One view (the membrane-mediated budding hypothesis) is that budding is an intrinsic property of Golgi membranes not requiring
T E Kreis et al.
Annual review of cell and developmental biology, 11, 677-706 (1995-01-01)
Cytosolic coat proteins (COPs) regulate membrane traffic in eukaryotic cells. Three classes of coat protein complexes have so far been identified: clathrin and its adaptor proteins, coatomer (COPI), and COPII. Coatomer (composed of seven different subunits) and ADP-ribosylation factor (ARF)
G J Choukroun et al.
The Journal of clinical investigation, 106(8), 983-993 (2000-10-18)
The Golgi complex and the trans-Golgi network are critical cellular organelles involved in the endocytic and biosynthetic pathways of protein trafficking. Lipids have been implicated in the regulation of membrane-protein trafficking, vesicular fusion, and targeting. We have explored the role
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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