G1549

PNGase F Elizabethkingia meningoseptica由来

ready-to-use solution, recombinant, expressed in E. coli

• 別名: N-グリコシダーゼF, PNGase F Chryseobacterium meningosepticum由来, PNGase F Flavobacterium meningosepticum由来, ペプチドN-グリコシダーゼ
• CAS番号: 83534-39-8
• Enzyme Commission number: 3.5.1.52 (BRENDA, IUBMB)
• MDL番号: MFCD00131222
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• リコンビナント: expressed in E. coli
• 品質水準: 200
• 結合体: (N-linked)
• グレード: Proteomics Grade
• フォーム: ready-to-use solution
• 比活性: ≥1000 U/mg
• シェルフライフ: ≥1 yr at -20 °C
• 分子量: ~36 kDa
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

アプリケーション

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

タンパク質の脱グリコシル化に使用できます。

生物化学的／生理学的作用

PNGase F from Elizabethkingia meningoseptica has glycan-binding catalytic domain and a bowl-like domain at the N-terminus. It cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It is cost-effectively produced on a large scale in prokaryotic hosts and requires divalent zinc ions for its enzymatic activity.

グリコシル化されたアスパラギン残基のN末端とC末端がポリペプチド鎖で置換されているとき、糖タンパク質からグリカン全体を切断します。

単位の定義

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

物理的形状

Supplied as 300 Units/mL enzyme in 50% (v/v) glycerol and 50% (v/v) 20 mM Potassium Phosphate, pH 7.5.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable