G1546

モノクローナル抗緑色蛍光タンパク質(GFP)抗体 マウス宿主抗体

clone GSN149, purified from hybridoma cell culture

• 別名: Mouse Anti-GFP
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: GSN149, monoclonal
• 形状: buffered aqueous solution
• 包装: antibody small pack of 25 μL
• テクニック: indirect ELISA: suitable; western blot: 1-2 μg/mL using extracts of cells expressing GFP fusion proteins
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

GFP融合タンパク質と特異的に反応します。

Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the hybridoma GSN149 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of the Green fluorescent protein from jellyfish Aequorea victoria. GFP is a 27 kDa protein (238 amino acids) that can produce a strong green fluorescence without the need for a substrate. It absorbs light maximally at 395 nm and emits a bright green fluorescence with a peak at 509 nm. The GFP chromophore is formed through cyclization and oxidation of an internal tripeptide motif (Ser⁶⁵, Gly⁶⁹ and Tyr⁶⁶).

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.

• Immunocytochemistry
• Immunofluorescence-cell culture cells
• Immunoprecipitation
• Western Blotting
• Western blotting following immunoprecipitation

Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse was used in surface plasmon resonance (SPR) gold chip analysis to study the cysteine- and domain-mediated immobilization of enhanced GFP model protein.

生物化学的／生理学的作用

GFP is implicated in protein detection and localization in living cells, as well as gene expression monitoring in prokaryotes and eukaryotes. It detects protein-protein interactions in living cells.

Green fluorescent protein, produced in the jellyfish, Aequorea victoria, acts as secondary fluorescent protein in an energy transfer reaction to produce green light. It ability to produce a highly visible, internal fluorophore is widely applicable as marker for gene expression and to produce chimeric proteins to understand protein biochemistry.

物理的形状

15 mMアジ化ナトリウム含有のpH 7.4の0.01 Mリン酸緩衝食塩水溶液.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)