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Merck
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主要文書

安全性情報

06-896

Sigma-Aldrich

Anti-GFP (Green Fluorescent Protein) Antibody

Upstate®, from chicken

別名:

Green fluorescent protein

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

chicken

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

polyclonal

交差性

vertebrates

メーカー/製品名

Upstate®

テクニック

immunocytochemistry: suitable
western blot: suitable

アイソタイプ

IgY

UniProtアクセッション番号

輸送温度

dry ice

ターゲットの翻訳後修飾

unmodified

詳細

Green fluorescent protein (GFP) is a 29 kDa fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488 nm), GFP strongly fluoresces (Emission max: 507 nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells.

特異性

Recognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins.

免疫原

His-tagged green fluorescent protein of Aequorea victoria.

アプリケーション

Research Category
エピトープタグ抗体及び凡用抗体
Research Sub Category
エピトープタグ
Anti-GFP (Green Fluorescent Protein) Antibody detects level of GFP (Green Fluorescent Protein) & has been published & validated for use in IC & WB.
Immunocytochemistry:
This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.

品質

Routinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.

ターゲットの説明

30 kDa

物理的形状

Ammonium Sulfate Precipitation and PEG
Format: Purified
Purified chicken polyclonal IgY from egg yolk in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

アナリシスノート

Control
Rat brain lysate, cells expressing GFP protein.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 1


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

06-896:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

MARK2/Par-1 guides the directionality of neuroblasts migrating to the olfactory bulb.
Sheyla Mejia-Gervacio,Kerren Murray,Tamar Sapir,Richard Belvindrah,Orly Reiner,Pierre-Marie Lledo
Molecular and Cellular Neurosciences null
Bcl-xL regulates metabolic efficiency of neurons through interaction with the mitochondrial F1FO ATP synthase.
Alavian, KN; Li, H; Collis, L; Bonanni, L; Zeng, L; Sacchetti, S; Lazrove, E; Nabili et al.
Nature Cell Biology null
LMO4 can interact with Smad proteins and modulate transforming growth factor-beta signaling in epithelial cells.
Lu, Z; Lam, KS; Wang, N; Xu, X; Cortes, M; Andersen, B
Oncogene null
Green fluorescent protein: applications in cell biology.
Gerdes, H H and Kaether, C
Febs Letters, 389, 44-47 (1996)
Distribution of nanoparticles throughout the cerebral cortex of rodents and non-human primates: Implications for gene and drug therapy.
Salegio, EA; Streeter, H; Dube, N; Hadaczek, P; Samaranch, L; Kells, AP; San Sebastian et al.
Frontiers in Neuroanatomy null

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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