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suspension
品質水準
シェルフライフ
1 yr
テクニック
western blot: suitable
Matrix
4% agarose
pH
7.2
キャパシティ
~10 μg(biotin per ml of packed gel)
輸送温度
wet ice
保管温度
2-8°C
詳細
The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
アプリケーション
Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
特徴および利点
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
物理的形状
PBS (50%グリセロ-ル, 15 ppm Kathon含有) 懸濁液1:1(v/v)。
法的情報
EZview is a trademark of Sigma-Aldrich Co. LLC
保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
E5529-5X1ML:
E5529-5ML:
E5529-1ML:
E5529-BULK:
E5529-VAR:
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関連コンテンツ
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
アフィニティーまたはGSTプルダウン法、タンデムアフィニティー精製（TAP）法、共免疫沈降法を用いてin vitroのタンパク質間相互作用を検討するためのプルダウンアッセイ、試薬、プロトコル
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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