E3406

抗EB1抗体 ウサギ宿主抗体

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-APC binding protein EB1, Anti-End-Binding-1, Anti-Microtuble-associated protein RP/EB family member 1
• MDL番号: MFCD01633308
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 35 kDa
• 交差性: rat, mouse, bovine, pig, canine, human
• 濃度: ~1.5 mg/mL
• テクニック: immunoprecipitation (IP): 10-20 μg using cell lysate of the human epitheloid carcinoma HeLa cell line; indirect immunofluorescence: 1-2 μg/mL using rat fibroblast Rat1 cells; indirect immunofluorescence: suitable; western blot: 0.5-1 μg/mL using cell lysate of the rat fibroblast cell line Rat1 or Rat 2
• UniProtアクセッション番号: Q15691
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MAPRE1(22919) ; mouse ... Mapre1(13589) ; rat ... Mapre1(114764)

詳細

詳細

Microtubule-associated protein RP/EB family member 1 is a protein encoded by the MAPRE1 gene in humans. EB1 (End-Binding-1) proteins belong to a distinct family of accessory proteins, termed “plus-end tracking proteins” or +TIPs, that bind to the plus-ends of microtubules. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.

免疫原

ヒトEB1のC末端に位置するアミノ酸252~268に相当する合成ペプチドのKLH結合体。この配列は複数の生物種で同一であり(マウス, ラット, イヌ, ウシ, ブタEB1)、ニワトリEB1で高度に保存(1アミノ酸置換)されていますが、ヒトEB2(同一性35%)、ヒトEB3(同一性53%)とのホモロジ-は限定的です。

アプリケーション

Anti-EB1 antibody produced in rabbit has been used in

• immunofluorescence
• western blotting
• immunocytochemistry
• immunoprecipitation

生物化学的／生理学的作用

End-Binding-1 (EB1) associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.

The C-terminal domain of End-binding proteins (EBs) is important for its autonomous microtubule tip tracking, regulation of microtubule dynamics, chromosome segregation and recruitment of numerous partners to microtubule ends. EB1 proteins bind to microtubule ends and act with the adenomatous polyposis coli (APC) tumor suppressor to regulate the microtubule filament system. It is a potential oncogene and plays an important role during early event in colon carcinogenesis, colorectal cancer (CRC). EB1 acts as a potential biomarker and therapeutic target in CRC.

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)