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由来生物
rabbit
品質水準
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
buffered aqueous solution
分子量
antigen ~80 kDa
交差性
human, rat
テクニック
immunohistochemistry: 20-40 μg/mL
immunoprecipitation (IP): 0.5-1 μg using RIPA extract of human A431 cells
indirect immunofluorescence: 10-20 μg/mL
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat NRK cells
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... EZR(7430)
mouse ... Ezr(22350)
rat ... Ezr(54319)
関連するカテゴリー
詳細
Ezrin is a 80kDa membrane-associated protein that belongs to ERM (Ezrin/Radixin/Moesin) family. Ezrin is found predominantly in polarized epithelial cells such as intestinal epithelial cells, gastric parietal cells, epithelial cells of the kidney proximal tubule, terminal bronchiole of the lung, and in mesothelia.
免疫原
synthetic peptide corresponding to amino acids 479-498 of human ezrin with N-terminal added cysteine, conjugated to KLH. The corresponding sequence in rat and mouse differs by three amino acids.
アプリケーション
Anti-Ezrin antibody produced in rabbit has been used in:
- immunolocalization studies
- immunostaining
- immunofluorescence and immunohistochemistry
- immunoblot
生物化学的／生理学的作用
Cultured neuronal cells do express ezrin to some extent, and whole brain tissue expresses low levels of ezrin. Ezrin expression is associated with malignancy in astrocytoma, osteosarcoma, mammary and pancreatic adenocarcinomas, and in cutaneous and uveal melanomas.
Ezrin promotes cell adhesion. The NH2-terminal domain negatively regulates membrane spreading and elongation properties of ezrin COOH-terminal domain. The members of ERM family are usually involved in cross-linking of plasma membrane and actin filaments. ERM proteins are involved in cell adhesion, cell shape determination, migration, and the organization of cell surface structures. They are also involved in signal transduction and apoptosis. Ezrin has been implicated in tumor progression and metastatic spread.
物理的形状
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
E1281-VAR:
E1281-200UL:
E1281-BULK:
IXO11696:
最新バージョンのいずれかを選択してください：
Vijay B Arumugham et al.
Frontiers in immunology, 9, 919-919 (2018-05-17)
A central feature of the immune synapse (IS) is the tight compartmentalization of membrane receptors and signaling mediators that is functional for its ability to coordinate T cell activation. Second messengers centrally implicated in this process, such as Ca2+ and
AICAR induces mitochondrial apoptosis in human osteosarcoma cells through an AMPK-dependent pathway
Morishita M, et al.
International journal of oncology, 50(1), 23-30 (2017)
Heat shock protein family B member 1 facilitates ezrin activation to control cell migration in esophageal squamous cell carcinoma
Xie YH, et al.
The International Journal of Biochemistry & Cell Biology, 112(19), 79-87 (2019)
Suvi Ilmonen et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 18(4), 503-510 (2004-10-12)
Ezrin is a member of the ezrin-radixin-moesin family of proteins that link the actin-containing cytoskeleton to the plasma membrane. Ezrin is also connected to signaling molecules involved in the regulation of cell survival, proliferation and migration. Here, we examined the
A Bretscher
Current opinion in cell biology, 11(1), 109-116 (1999-02-27)
Molecules involved in ERM (ezrin-radixin-moesin) based attachment of membrane proteins to the cortical cytoskeleton in cell surface structures have been identified. In lymphocytes, a direct interaction is seen with extracellular matrix receptors and intercellular adhesion molecules. In polarized epithelial cells
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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