AB3832

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific

Chemicon^®, from rabbit

• 別名: Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: monkey, human, mouse, rat
• メーカー／製品名: Chemicon^®
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NM_004252.2
• UniProtアクセッション番号: O14745
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: phosphorylation (pThr567)
• 遺伝子情報: human ... SLC9A3R1(9368)

詳細

特異性

Recognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin, and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1.

免疫原

Epitope: phospho-specific

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin

アプリケーション

Research Category
細胞骨格

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.

Research Sub Category
Cytoskeleton

Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight). The antibody recognizes 75KDa (Moesin, and ~80kDa Ezrln & Radixin) bands.

Immunohistochemistry (paraffin): 1:50

Optimal working dilutions must be determined by the end user.

ターゲットの説明

75KDa Moesin, and ~80kDa Ezrin & Radixin

物理的形状

ImmunoAffinity Purified

Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.

保管および安定性

Maintain at -20°C for 12 months, do not aliquot.

アナリシスノート

Control
Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2