AB3843

Anti-Ezrin/Radixin/Moesin Antibody

Chemicon^®, from rabbit

• 別名: Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, monkey, rat, human
• メーカー／製品名: Chemicon^®
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NM_004252.2
• UniProtアクセッション番号: O14745
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SLC9A3R1(9368)

詳細

特異性

Recognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton, and are involved in cell adhesion, membrane ruffling, and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1.

免疫原

KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.

アプリケーション

Research Category
細胞骨格

Research Sub Category
接着分子（CAM）

Detect Ezrin/Radixin/Moesin using this Anti-Ezrin/Radixin/Moesin Antibody validated for use in WB, IC, IH(P).

Western blotting: 1:1,000. The 75 kDa band is Moesin and the 80kDa band is Ezrin and Radixin. (For best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)

Immunohistochemistry (Paraffin): 1:50. Positive control tissue is breast carcinoma. Cellular localization is membrane and cytoplasm

Immunocytochemistry (IF): 1:100

Optimal working dilutions must be determined by the end user.

物理的形状

Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.

保管および安定性

Maintain at -20ºC for 12 months, do not aliquot.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2