AB2033

Anti-Fibronectin Antibody

Chemicon^®, from rabbit

• 別名: Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; radioimmunoassay: suitable
• NCBIアクセッション番号: NM_002026.2
• UniProtアクセッション番号: P02751
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Fn1(14268)

詳細

詳細

Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma. The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane-bound fibronectin receptors on cell surfaces. The immunogen for fibronectin polyclonal antibody AB2033 is purified mouse plasma. The antibody shows less than 0.1% reactivity with mouse laminin, and collagen types I, III, and IV by radioimmunoassay.

特異性

This antibody reacts with mouse fibronectin. Shows less than 0.1% reactivity with mouse Laminin, and Collagen Types I, III, and IV by radioimmunoassay.

免疫原

Fibronectin extracted and purified from mouse plasma.

アプリケーション

Research Category
細胞骨格

Research Sub Category
ECMタンパク質

Detect Fibronectin using this Anti-Fibronectin Antibody validated for use in ELISA, IC, IH, RIA.

Immunohistochemistry:
1:80 dilution of a previous lot was used in immunofluorescent staining of frozen mouse skin and liver tissues.

Radioimmunoassay:
A previous lot of this antibody was used in RIA.

ELISA:
A 1:200 dilution of a previous lot was used in ELISA.

Optimal working dilutions must be determined by the end user.

品質

Evaluated by immunocytochemistry on NIH/3T3 cells.

Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of NIH/3T3 using AB2033 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue).

物理的形状

ImmunoAffinity Purified

The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µg/ml of antibiotics and antimycotics

保管および安定性

Stable for 1 year at -20ºC from date of receipt.

アナリシスノート

Control
Extracellular matrix in most tissue

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable