D9281

λDNA , EcoR I Hind III 消化

for DNA electrophoresis

• UNSPSCコード: 41141827
• NACRES: NA.25

特性

• 品質水準: 100; 200
• 形状: liquid
• 使用法: 100 uses (0.1 mg size); 500 uses (0.5 mg size)
• 適合性: suitable for electrophoresis (DNA)
• 保管温度: −20°C

詳細

詳細

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

アプリケーション

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

構成

10mM Tris-HCl溶液 (pH 8.0, 1mM EDTA含有)

その他情報

125–21,226 bpの13フラグメントを含有

For optimal resolution, the recommended agarose gel concentration is 0.7%.

調製ノート

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable