D6293

pUC18 DNA HaeIII 消化

for DNA electrophoresis

• UNSPSCコード: 12352202
• NACRES: NA.25

特性

• 形状: liquid
• 使用法: 200 uses (0.1 mg size); 40 uses (20 μg size)
• 適合性: suitable for electrophoresis (DNA)
• 保管温度: −20°C

詳細

詳細

Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

アプリケーション

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.

構成

10mM Tris HCl(pH 8.0, 1mM EDTA含有)

その他情報

11~587 bpの11個の断片を含んでいます。

For optimal resolution, the recommended agarose gel concentration is 2.5%.

調製ノート

Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable