L6.C11

92102119, rat muscle, Fibroblast

• UNSPSCコード: 41106514
• 由来生物: rat muscle
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified

特性

• 製品名: L6.C11, 92102119
• 由来生物: rat muscle
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat skeletal muscle myoblast

細胞株の説明

A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned.

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.

その他情報

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