1306

90011887, human skin, Fibroblast

• UNSPSCコード: 41106514

特性

• 製品名: 1306, 90011887
• 由来生物: human skin
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human skin fibroblast

細胞株の説明

A HPRT- derivative of the SV40 transformed human fibroblast line GM0637. The cells were selected in 5μg/ml thioguanine and are useful for transfection experiments.

アプリケーション

Transfection studies

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 13
D16S539: 12,13
D5S818: 11
D7S820: 10
THO1: 7,9
TPOX: 9,11
vWA: 16,17

培地

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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