RK-13 Cell Line from rabbit

21715, rabbit kidney, Epithelial-like

• 別名: GL RK/13 Cells, Glaxo Laboratories Rabbit Kidney 13 Cells, R13 Cells
• UNSPSCコード: 41106514
• eCl@ss: 32190102

特性

• product name: RK-13 Cell Line from rabbit, rabbit kidney, 00021715
• 由来生物: rabbit kidney
• 成長モード: Adherent
• 核型: 2n=44, stem-line = 56 (=22%)
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rabbit kidney

細胞株の説明

RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.

アプリケーション

Virus studies

培地

EMEM(HBSS) (M5775) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.

その他情報

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