CAS9D10ARFPP

CAS9D10A プラスミド

• 別名: CAS9D10A Plasmid
• UNSPSCコード: 41106609
• NACRES: NA.51

特性

• リコンビナント: expressed in E. coli
• 品質水準: 200
• 包装: vial of 50 μL
• 濃度: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
• プロモーター: Promoter name: CMV
• レポーター遺伝子: RFP
• セレクション: kanamycin
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

The Cas9-D10A nickase plasmid co-expressing RFP uses the CMV promoter for strong transient expression of Cas9-D10A. Alternate promoters can be substituted by replacement of CMV using MluI and NheI. Also, the Cas9D10A expression plasmids can be linearized using XbaI for T7-based mRNA production.

アプリケーション

Functional Genomics
Use of Paired Cas9 Nickase + RFP for:

• Creation of gene knockouts in multiple cell lines
• Complete knockout of genes not amenable to RNAi
• Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes

構成

1 vial containing 1ug of Cas9-D10A Nickase-RFP plasmid.

Please note, this product does not contain any guide RNA sequence. A pair of gRNA plasmids must be purchased separately through the Custom CRISPR paired nickase product tab.

物理的形状

2 ug of Sigma Cas9-D10A nickase-RFP plasmid

その他情報

The type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. RFP is co-expressed from the same mRNA as the Cas9 nickase protein via a 2A peptide linkage, enabling tracking of transfection efficiency and enrichment of genome editing activity in cell populations via fluorescence activated cell sorting (FACS).

法的情報

CRISPR Use License Agreement

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable