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由来生物
bacterial (Clostridium histolyticum)
品質水準
形状
solid
比活性
≥800 CDU/mg solid
2-5 FALGPA units/mg solid
分子量
68-130 kDa
輸送温度
dry ice
保管温度
−20°C
遺伝子情報
rat ... LOC296948(296948)
アプリケーション
Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.
生物化学的／生理学的作用
Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca2+) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn2+ is required for activity. This product is used if the collagenase does not require a significant protease activity.
その他情報
膵臓組織におけるトリプシン酵素活性を制限するため、プロテア-ゼインヒビタ-を混合した、厳選したロットのコラゲナ-ゼType XIです。 この組成物は、いくつかの社外研究機関との共同研究により開発されました。
品質
クロストリパイン活性および非特異的中性プロテア-ゼ活性も有しています。
阻害剤
製品番号
詳細
価格
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C6079-BULK:
C6079-1G:
C6079-500MG-PW:
C6079-5G:
C6079-500MG:
C6079-5G-PW:
C6079-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Beverly Duncan et al.
PloS one, 5(7), e11427-e11427 (2010-07-14)
Double negative CD3(+)4(-)8(-) TCR alphabeta splenic cells (DNCD3) can suppress the immune responses to allo and xenografts, infectious agents, tumors, and some autoimmune disorders. However, little is known about their role in autoimmune diabetes, a disease characterized by the reduction
S P Gupta et al.
Bioorganic & medicinal chemistry, 11(14), 3065-3071 (2003-06-24)
A quantitative structure-activity relationship (QSAR) study has been made on eight different series of Clostridium histolyticum collegenase (ChC) inhibitors. These series are comprised of four different groups of sulfonylated amino acids and their corresponding hydroxamates. In each series, the inhibition
Association of immune system gene polymorphisms with quantitative features which are pathogenetically important in chronic viral hepatitis.
Goncharova, I., et al.
Molecular Biology, 42, 242-246 (2008)
C J Swanson et al.
Human immunology, 62(7), 739-749 (2001-06-26)
Recent progress in human islet transplantation demonstrates the feasibility of using purified human islets for treatment of type 1 diabetes mellitus; however, a shortage of human pancreata remains a major obstacle. This report describes methods to isolate porcine islets using
Brandon M Blobner et al.
American journal of physiology. Gastrointestinal and liver physiology, 321(5), G449-G460 (2021-09-16)
Chronic pancreatitis (CP) is a complex inflammatory disorder with numerous associated genetic and environmental risk factors. The most distressing characteristic of CP is recalcitrant pain, often requiring surgical resection including total pancreatectomy with islet autotransplantation (TPIAT). We studied five consented
プロトコル
This procedure may be used for Collagenase products.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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