C1956

モノクロナール抗カルシニューリン (α-サブユニット) マウス宿主抗体

clone CN-A1, ascites fluid

• MDL番号: MFCD00282168
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: CN-A1, monoclonal
• 含みます: 15 mM sodium azide
• 化学種の反応性: human, bovine, rat
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using neurons in human ganglia; indirect ELISA: suitable; microarray: suitable; western blot: 1:10,000 using rat brain extract
• アイソタイプ: IgG1
• UniProtアクセッション番号: P63098
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPP3R1(5534) ; rat ... Ppp3r1(29748)

詳細

詳細

Calcineurin (CaN)(heterodimeric enzyme) is a Ca²⁺/calmodulin (CaM)-stimulated protein phosphatase 3 (PPP3) consisting of a catalytic A subunit (CnA) and a Ca²⁺-binding regulatory B subunit (CnB). The calcium-binding regulatory subunit (calcineurin B) is coded by the PPP3R1 gene located on human chromosome 2. Protein phosphatase 3, regulatory subunit B, α (PPP3R1) is also known as CALN, CCN1, CNA1, CALNA, PPP2B and CALNA1. The regulatory subunit calcineurin B is widely expressed in brain while the catalytic subunit (calcineurin A) is abundantly expressed in Hassall′s corpuscles, localized in the thymic medulla and represents the terminal stages of thymic medullary epithelium.

Monoclonal Anti-Calcineurin (α-subunit) (mouse IgG1 isotype) is derived from the CN-A1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.

特異性

この製品は、イムノブロッティングにおいてカルシニュ-リンのα-サブユニット(61 kDa、別名カルシニュ-リンA)上のエピト-プを認識しますが、β-サブユニットとは交差反応しません。

免疫原

ウシ脳カルシニュ-リン

アプリケーション

Monoclonal Anti-Calcineurin (α-Subunit) antibody produced in mouse has been used in:enzyme linked immuno sorbent assay (ELISA) ,immunohistochemistry, western blotting

生物化学的／生理学的作用

Calcineurin is a major soluble calmodulin-binding protein in the brain. It stimulates the expression of the surface molecules CD83, CD80, CD86, CD40 and HLA-DR by promoting secretion of inflammatory cytokines IL-6, TNF-α and IL-1β by human PBMC-derived dendritic cells. It plays an important role in signal transduction, activation of T cell. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable